Transcriptomics

Dataset Information

178

MicroRNA profiling of human papillary thyroid carcinoma and normal thyroid to assess the role of miRNA genes in papillary thyroid carcinoma


ABSTRACT: Apart from alterations in the RET/PTC-RAS-BRAF pathway, comparatively little is known about the genetics of papillary thyroid carcinoma (PTC). We show that numerous miRNAs are transcriptionally up-regulated in PTC tumors compared with unaffected thyroid tissue. A set of 5 miRNAs including the 3 most upregulated ones (miRs 221, 222, 146) distinguished unequivocally between PTC and normal thyroid. Additionally, miR-221 was upregulated in unaffected thyroid tissue in several PTC patients, presumably an early event in carcinogenesis. Tumors in which the upregulation (11-19 fold) of miRs 221, 222 and 146 was strongest showed dramatic loss of KIT transcript and Kit protein. In five of 10 such cases this was associated with germline single nucleotide changes in the two recognition sequences in KIT for these miRNAs. We conclude that upregulation of several miRs and down regulation of KIT are involved in PTC pathogenesis, and that sequence changes in genes targeted by miRNAs can contribute to their downregulation.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Homo sapiens  

SUBMITTER: Saul Suster   Carlo M. Croce  Krystian Jazdzewski  Kaarle Franssila  Rebecca Nagy  George A. Calin  Wei Li  Richard T. Kloos  Stefano Volinia  Chang-gong Liu  Albert de la Chapelle  Sandya Liyanarachchi  Huiling He 

PROVIDER: E-TABM-68 | ArrayExpress | 2005-02-28

REPOSITORIES: ArrayExpress

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Publications

The role of microRNA genes in papillary thyroid carcinoma.

He Huiling H   Jazdzewski Krystian K   Li Wei W   Liyanarachchi Sandya S   Nagy Rebecca R   Volinia Stefano S   Calin George A GA   Liu Chang-Gong CG   Franssila Kaarle K   Suster Saul S   Kloos Richard T RT   Croce Carlo M CM   de la Chapelle Albert A  

Proceedings of the National Academy of Sciences of the United States of America 20051219 52


Apart from alterations in the RET/PTC-RAS-BRAF pathway, comparatively little is known about the genetics of papillary thyroid carcinoma (PTC). We show that numerous microRNAs (miRNAs) are transcriptionally up-regulated in PTC tumors compared with unaffected thyroid tissue. A set of five miRNAs, including the three most up-regulated ones (miR-221, -222, and -146), distinguished unequivocally between PTC and normal thyroid. Additionally, miR-221 was up-regulated in unaffected thyroid tissue in sev  ...[more]

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