Transcription profiling by array of Enterococcus faecalis V583 spontaneous pediocin PA-1 mutants MOP1, MOP2 and MOP5 and delta-mptD mutant MOM1 to study class IIa bacteriocin resistance
ABSTRACT: The transcriptional responses of class IIa bacteriocin resistance in 4 mutants of Enterococcus faecalis V583; three spontaneous pediocin PA-1 mutants; MOP1, MOP2 and MOP5 and one delta-mptD mutant called MOM1, studied by microarrays in E. faecalis V583.
The class IIa bacteriocin, pediocin PA-1, has clear potential as food preservative and in the medical field to be used against Gram negative pathogen species as Enterococcus faecalis and Listeria monocytogenes. Resistance towards class IIa bacteriocins appear in laboratory and characterization of these phenotypes is important for their application. To gain insight into bacteriocin resistance we studied mutants of E. faecalis V583 resistant to pediocin PA-1 by use of transcriptomic analyses.Mutan ...[more]
Project description:Gene expression of Enterococcus faecalis V583 in response to growth in 10% and 100% blood was examined by the use of of genome wide microarrays. Transcriptional profiles were obtained through time series experiments over periods of 60min (for 10% blood) and only after 30 min for growth 100% blood.
Project description:The effects of bovine bile and SDS on transcriptional events were studied by means of genome wide microarrays in Enterococcus faecalis V583. Transcriptional profiles were obtained through time series experiments over periods of 120min for cells treated with bile and the combination of SDS and bile, and 360min. for SDS.
Project description:Differences in gene content between three test strains were first assessed by comparative genomic hybridization using an E. faecalis V583 oligo array and with E. faecalis V583 as a reference strain . Transcriptional profiles of the same three test strains were then obtained through time series experiments over periods of 30min.
Project description:Gene content in various Enterococcus faecalis strains compared to E. faecalis V583. Strains have been compared to the V583 strain by comparative genomic hybridization using genome-wide PCR-based microarrays representing the V583 genome. Genes have been deemed "present" or "divergent" in the various strains.
Project description:Comparative genomic hybridization of 9 Norwegian E. faecalis baby isolates with E. faecalis V583 as a reference strain using an E. faecalis V583 oligo array. Total gene content was analyzed by whole genome microarrays.
Project description:The effects of NaCl on transcriptional events were studied by means of genome wide microarrays in Enterococcus faecalis V583. Transcriptional profiles were obtained through time series experiments over periods of 60min.
Project description:Trancriptional response of pediocin exposure of V583 and V583 expressing immunity (petAIM), and the transcriptional response of petAIM compared to both V583 and V583 containing the vector pet.
Project description:The aim was to study the transcriptional profiling of the E. faecalis V583 in presence and absence of tyrosine. We compared the expression profile of the strains grown in M17 medium with glucose as carbon source (GM17) and suplemented with agmatine in presence and absence of tyrosine. Overall design: E. faecalis V583 cells grown with 15 mM tyrosine (test) compared with E. faecalis V583 cells grown without tyrosine (reference). Both strains grown in GM17 medium suplemented with 5 mM agmatine.