To survive in a host environment, microbial pathogens must sense local conditions, including nutrient availability, and adjust their growth state and virulence functions accordingly. No comprehensive investigation of growth phase-related gene regulation in Bordetella pertussis has been reported previously. We characterized changes in genome-wide transcript abundance of B. pertussis as a function of growth phase and availability of glutamate, a key nutrient for this organism. Using a Bordetella D ...[more]
Project description:B. pertussis GMT-1 was grown in media containing magnesium sulfate to put the culture in the Bvg- phase. The cells were then washed and grown in media lacking magnesium sulfate and samples were harvested at time=0 and several time points beyond.
Project description:Two strains of B. pertussis were serially passaged on plates in the laboratory. Global gene expression profiles were measured for passage 1 and late-passage cultures grown in Bvg+ and Bvg- environmental conditions.
Project description:B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until three hours beyond the log to stationary phase transition.
Project description:B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until stationary phase. Samples were taken at multiple points along the growth curve.
Project description:bvgS phase-locked mutant derivatives of B. bronchiseptica RB50, B. pertussis GMT-1, and B. pertussis Tohama I were grown at 37 degrees C in the absence of modulator and harvested at mid-log phase. Comparison of gene expression profiles identified a set of genes in each strain that were regulated by BvgAS.
Project description:Three B. bronchiseptica strains were grown at 37 degrees C in media containing or lacking nicotinic acid. Samples were harvested at mid-log phase and gene expression difference between the treated and untreated was measured for each strain.