Transcriptomics

Dataset Information

36

Transcription profiling by array of mouse mammary epithelial stem cell tumours.


ABSTRACT: The aim of the experiment was to analyse gene expression profiles in Brca1 tumours arising from different mammary epithelial cell populations use a Cre-loxP based conditional knockout system. K14 promoter driving Cre expression caused Brca1 knockout in basal stem cells and thus stem cell origin tumours whereas Blg promoter driving Cre expression caused Brca1 knockout in luminal progenitor cells and thus progenitor origin tumours. Individual arrays were carried out on labelled cDNA made from RNA isolated from mouse mammary tumours. Only cDNA passing Almac diagnostics QC criteria were hybridised to arrays. Only arrays passing QC criteria after hybrisiation were subsequently analysed.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

DISEASE(S): Invasive Ductal Carcinoma,Metaplastic Spindle Cell Carcinoma,Malignant Adenomyoepithelioma,Mammary Tumour Of Unknown Subtype,Invasive Ductal Carcinoma With Metaplastic Elements

SUBMITTER: Anita Grigoriadis   

PROVIDER: E-TABM-997 | ArrayExpress| 2014-05-02

REPOSITORIES: ArrayExpress

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Publications

Embryonic mammary signature subsets are activated in Brca1-/- and basal-like breast cancers.

Zvelebil Marketa M   Oliemuller Erik E   Gao Qiong Q   Wansbury Olivia O   Mackay Alan A   Kendrick Howard H   Smalley Matthew J MJ   Reis-Filho Jorge S JS   Howard Beatrice A BA  

Breast cancer research : BCR 20130318 2


INTRODUCTION: Cancer is often suggested to result from development gone awry. Links between normal embryonic development and cancer biology have been postulated, but no defined genetic basis has been established. We recently published the first transcriptomic analysis of embryonic mammary cell populations. Embryonic mammary epithelial cells are an immature progenitor cell population, lacking differentiation markers, which is reflected in their very distinct genetic profiles when compared with th  ...[more]

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