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Omics score: 2

Transcription profiling of Arabidopsis Umkirch-1/Umkirch-3 hybrid plants compared to siblings carrying a microRNA targeting the Umkirch-3 allele of At5g41750

ABSTRACT: Umkirch-1/Umkirch-3 hybrid plants (KB142-2) grown at 16 degreeC long day were compared with siblings carrying a microRNA targeting the Umkirch-3 allele of At5g41750. Plants were about 3 weeks old, and whole rosettes were harvested. Each sample is a pool of 8-10 individuals.

PROVIDER: E-ATMX-26 | ExpressionAtlas | 2015-08-27

REPOSITORIES: ExpressionAtlas

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Autoimmune response as a mechanism for a Dobzhansky-Muller-type incompatibility syndrome in plants.

Bomblies Kirsten K Lempe Janne J Epple Petra P Warthmann Norman N Lanz Christa C Dangl Jeffery L JL Weigel Detlef D

PLoS biology 20070901 9

Epistatic interactions between genes are a major factor in evolution. Hybrid necrosis is an example of a deleterious phenotype caused by epistatic interactions that is observed in many intra- and interspecific plant hybrids. A large number of hybrid necrosis cases share phenotypic similarities, suggesting a common underlying mechanism across a wide range of plant species. Here, we report that approximately 2% of intraspecific crosses in Arabidopsis thaliana yield F1 progeny that express necrosis ...[more]

PMID: 17803357

Similar Datasets

Transcription profiling of Arabidopsis Umkirch-1/Umkirch-3 hybrid plants compared to siblings carrying a microRNA targeting the Umkirch-3 allele of At5g41750

Project description:Umkirch-1/Umkirch-3 hybrid plants (KB142-2) grown at 16 degreeC long day were compared with siblings carrying a microRNA targeting the Umkirch-3 allele of At5g41750. Plants were about 3 weeks old, and whole rosettes were harvested. Each sample is a pool of 8-10 individuals.

2007-07-11 | E-ATMX-26 | biostudies-arrayexpress

RNAseq of Arabidopsis plants lacking E2 ubiquitin conjugating enzymes UBC35 and UBC36

Project description:This RNAseq experiment aims at determining the reprogramming of gene expression upon loss of K63 polyubiquitin chain formation. Twelve-day-old plants expressing a beta-estradiol inducible artificial microRNA targeting the two plant E2 enzymes catalyzing K63 polyubiquitin chain formation were mock-treated or beta-estradiol-treated (5microM). Total RNAs from whole seedings were extracted and subjected to RNA-seq analyses. Genes that are differentially regulated between non-treated (uninduced) and treated (induced with beta-estradiol) were identified.

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Small RNA sequencing in Arabidopsis plants

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2013-12-22 | GSE36934 | GEO

Response of potato and tobacco plants to photoperiod changes

Project description:Plants of Solanum tuberosum ssp andigena, which tuberize only under SD conditions, were grown in growth chambers under non-inductive LD conditions and half of the plants were transferred to inductive SD conditions for 1 or 15 days, whilst the rest of the plants were kept as controls in LD. Transgenic potato plants with reduced phyB levels, which tuberize irrespective of photoperiod, were also grown in LD conditions. All the plants, irrespective of treatment, were harvested 14 hours after the beggining of the photoperiod (i.e. immediately before dusk for plants on LD, and 8 hours after dusk for the plants on SD). A similar experimental protocol was used with plants of N. tabacum cv Hicks that flower at the same time irrespective of photoperiod and the isogenic line N. tabacum cv Hicks carrying the Maryland Mammoth mutant allele, which flower only on SD (i.e. the plants were grown under LD for 55 or 41 days in LD and then transferred for 1 or 15 days to SD conditions, respectively, whilst control plants were grown 56 days in LD). Finally, Nicotiana silvestris plants that flower only on LD were grown in non-inductive SD and then half of the plants were transferred to inductive LD conditions for 1 or 15 days. In all cases we harvested only the leaves, the organ in which daylength perception takes place and photoperiodic responses are initiated. SD conditions in this experiments were 8 hours of light/ 16 hours of darkness, 160 uEinstein, 22 °C. LD conditions were 16 hours of light/ 8 hours of darkness, 80 uEinstein, 22 °C. RNA was extracted with TRIZOL. Keywords: Direct comparison / Balanced Block Design

2007-09-01 | GSE8142 | GEO

Small RNA sequencing in Arabidopsis plants

Project description:Identification of microRNA expressed in Arabidopsis plants grown at different ambient CO2 concentrations and different ambient temperatures Small RNA sequencing of Arabidopsis wild type ecotype Columbia (Col-0) rossette leaves at bolting stage grown at 430 M-BM-1 50ppm and 810 M-BM-1 50ppm CO2 concentrations and 22 M-BM-1 0.5oC and 28 M-BM-1 0.5oC temperatures

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RNA-seq data of three Lupinus angustifolius lines profiled for Diaporthe toxica resistance, based on stem RNA isolates from inoculated and control plants

Project description:Very resistant line (75A:258, carrying the Phr1 allele), moderately resistant line (Wonga, carrying the PhtjR allele) and a susceptible line (Emir) were used in this study. 4 weeks after plant sowing lower parts of stems were scarified and inoculated with Diaporthe toxica (studied plants) or not inoculated (control plants). Stem tissue was sampled at 1, 5, 9, 16 and 23 days post inoculation.

2021-01-11 | E-MTAB-9814 | biostudies-arrayexpress

RNA-seq data of four Lupinus angustifolius lines profiled for anthracnose resistance, based on leaves RNA isolates from inoculated and control (mock-inoculated) plants

Project description:Resistant lines (Mandelup, carrying the AnMan allele and 83A:476 carrying the Lanr1 allele), the line which is putative novel donor of resistance (Boregine) and a susceptible line (Population 22660) were used in this study. 4 weeks after sowing, when the plants reached 4-6 leaf stage, plants were inoculated with Colletotrichum lupini or mock-inoculated (control plants) and then kept for 24 hours in darkness under ~100% humidity and temperature of 25°C. Leaves were sampled at 6, 12, 24, 36, and 48 hours post inoculation.

2022-11-02 | E-MTAB-11164 | biostudies-arrayexpress

Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants

Project description:Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants with constitutive expression of the chimeric receptors NRG1 grown at different temperature To reveal the underlying molecular mechanism of de-cosuppression with memory by high temperature in Arabidopsis, we performed the expression profiles of microRNA and SiRNA in transgenic plants with constitutive expression of the chimeric receptors NRG1 and wide type Col-0 grown at different temperature using the Custom LC Sciences Arabidopsis microRNA and SiRNA array. Keywords: high temperature, de-cosuppression, MicroRNA, SiRNA

2008-05-24 | GSE11535 | GEO

Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with different LFY orthologs to investigate evolutionary divergence of LFY function

Project description:The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from flowering and non flowering species to complement an Arabidopsis LFY mutant. Plants expressing, in a homozygous lfy12 background, the open reading frame of LFY orthologs under the control of Arabidopsis LFY promoter were synchronously induced to flower by growing plants in short days for 30 days then shifting them to Long Day for an additional 8 days. Shoot apices were dissected at either d0 or d8 in long days. Two biological replicates were performed. The following genotypes were used: Col - wild type arabidopsis; reference strain LFY (lfy12; LFY::AthLFY) - Arabidopsis UNI (lfy12; LFY::UNI) - Pisum ALF (lfy12; LFY::ALF) - Petunia WelNDLY (lfy12; LFY::WelNDLY) - Welwitschia mirabilis CrLFY1 (lfy12; LFY::CrLFY1) - Ceratopteris richardii CrLFY2 (lfy12; LFY::CrLFY2) - Ceratopteris richardii lfy-2; weak lfy allele lfy-9; intermediate leafy allele lfy-12; strong leafy allele.

2009-07-23 | E-MEXP-2258 | biostudies-arrayexpress

Transcription profiling of Arabidopsis Bla-1/Hh-0 hybrids, and Mir-0/Se-0 hybrids and their parents grown at 16C in long day conditions

Project description:Bla-1, Hh-0 and their F1 hybrid, and Mir-0, Se-0 and their F1 hybrid were grown for 3 weeks at 16 degreC long days. Whole rosettes were harvested and pooled 8-10 per sample.

2007-07-11 | E-ATMX-27 | biostudies-arrayexpress

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