Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-11258

ABSTRACT: RNAi profiling of mouse hippocampal neurons infected with lentivirus expressing Npas4-RNAi or control-RNAi to study Npas4-regulated genes

PROVIDER: E-GEOD-11258 | ExpressionAtlas | 2020-04-03

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Similar Datasets

RNAi profiling of mouse hippocampal neurons infected with lentivirus expressing Npas4-RNAi or control-RNAi to study Npas4-regulated genes

Project description:We performed a DNA microarray experiment to identify activity-regulated genes that are misregulated in the absence of Npas4. Experiment Overall Design: We infected mouse hippocampal neurons with lentivirus expressing Npas4-RNAi or control-RNAi @ 3 DIV and depolarized the neurons @ 8 DIV with 50 mM of KCl for 0, 1, 3 or 6 hours. Neurons were lysed, mRNA isolated and hybridized to Affymetrix arrays. Data were collected from 3 independent experiments.

2008-10-26 | E-GEOD-11258 | biostudies-arrayexpress

Mus musculus

Project description:Npas4-regulated genes in mouse hippocampal neurons

| PRJNA109195 | ENA

Effect of the combined loss of tomosyn (STXBP5) and tomosyn-2 (STXBP5L) on the proteome of primary mouse hippocampal neurons

Project description:Tomosyn (STXBP5) is a non-canonical SNARE protein enriched in many secretory cells and implicated in regulation of exocytosis. In neurons, loss of tomosyn affects fusion of synaptic vesicles. Here, we examined the impact of the loss of tomosyn (STXBP5) and its close paralog, tomosyn-2 (STXBP5L), on the proteome of primary mouse hippocampal neurons. To do this, we used a mouse model carrying floxed alleles of Stxbp5 and Stxbp5l genes. Primary hippocampal neurons isolated from these mice were transduced with Cre-recombinase, which expression resulted in a nearly complete loss of the two genes expression. As control, we used neurons from the same culture preparation but expressing Cre-recombinase lacking DNA-binding domain (ΔCre). This dataset provides the comparison of proteome of control ('WT') and Stxbp5/5l double knockout ('DKO') neurons.

2022-12-19 | PXD038442 | Pride

Mus musculus

Project description:Identification of synaptic activity-regulated genes in primary mouse hippocampal neurons

| PRJNA357078 | ENA

Transcription profiling of mouse primary cultured neurons to identify activity related gene expression

Project description:This SuperSeries is composed of the following subset Series:; GSE11256: KCl depolarization-regulated genes in mouse cortical neurons; GSE11258: Npas4-regulated genes in mouse hippocampal neurons Experiment Overall Design: Refer to individual Series

2008-10-26 | E-GEOD-11261 | biostudies-arrayexpress

Project description:Study of activity-regulated genes in mouse primary cultured neurons

| PRJNA106775 | ENA

A comparative transcriptomics approach unveils networks of activity-driven neuronal gene expression

Project description:Primary cultured hippocampal neurons were infected with lentiviral particles bearing constitutively active versions of the transcription factors CREB, SRF, EGR1 and FOS to define the downstream genetic program associated with each of these paradigmatic transcription factors in neurons. For CREB, we also extracted samples at a shorter infection time to try to dissect the different components of the wider genetic response observed upon expression of a constitutively active version of this transcription factor. In order to further explore the contribution of these transcriptional regulators to activity-driven gene expression, we also treated cultured hippocampal neurons infected with GFP-only expressing lentiviral particles with different drugs (bicuculline, forskokine, and BDNF) and profiled the associated transcriptional response. Finally, in order to further explore the role of CREB in forskolin-mediated gene expression, we also used A-CREB, a dominant negative form of this transcription factor. Specifically, we stimulated neurons with forskolin in the presence or absence of lentiviral particles expressing A-CREB and carried out genome-wide transcriptional profiling.

2011-11-19 | E-MEXP-3167 | biostudies-arrayexpress

Genome-wide analysis of T3 and CORT dependent gene expression and hormone synergy in mouse hippocampal neurons (HT-22)

Project description:Analysis of T3 and CORT dependent gene expression tested in the mouse hippocampal neuronal cell line HT-22. The experiment was done to identify genes which are differentially regulated by the two hormones as well as to identify genes which are synergistically regulated by combined T3 + CORT treatment. Results provide an exploratory dataset for studying T3 and CORT regulation in mouse hippocampal neurons.

2019-07-18 | GSE132423 | GEO

Deficiency of intellectual disability-related gene Brpf1 attenuated hippocampal excitatory neurotransmission and impaired learning and memory behavior

Project description:We hypothesized that mouse Brpf1 plays critical roles in the morphology and function of hippocampal neurons, and its deficiency leads to learning and memory deficits. To test this, we performed immunofluorescence, whole-cell patch clamp, mRNA-Seq on shBrpf1 infected primary cultured hippocampal neurons to study the effect of Brpf1 knockdown on neuronal morphology, electrophysiological characteristics and gene regulation. In addition, we performed stereotactic injection into adult mouse hippocampus to knockdown Brpf1 in vivo and examined the learning and memory ability by Morris Water Maze. We found that mild knockdown of Brpf1 reduced mEPSC frequency of cultured hippocampal neurons, before any significant changes of dendritic morphology showed. We also found that hippocampal knockdown of Brpf1 reduced the learning and memory ability of mice to some extent. Finally, mRNA-Seq analyses showed that genes related to learning, memory, movement and synaptic transmission (such as C1ql1, Gpr17, Htr1d, Glra1, Cxcl10, Grin2a) were dysregulated upon Brpf1 knockdown. Our results showed that Brpf1 mild knockdown attenuated hippocampal excitatory neurotransmission and reduced learning and memory ability, which helps explain the symptoms of patients with BRPF1 mutations.

2021-05-19 | GSE174600 | GEO

shRNA profiling of rat neurons to identify MEF2 Regulated Genes

Project description:Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2; Experiments were conducted to identify activity-regulated MEF2 target genes. Experiment Overall Design: Neurons were depolarized with 55mM extracellular KCl for one or six hours in the presence of control or MEF2-specific shRNAs

2009-01-17 | E-GEOD-13537 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data