Project description:Purpose: The purpose of this experiment was to identify the gene expression changes in Arabidopsis thaliana seedlings induced by 1 h of 100 µM NAE 18:3 treatment. Methods: mRNA profiles of 4-day-old Arabidopsis thaliana seedling were generated by deep sequencing, in triplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed at the transcript level using TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays
Project description:Monitor changes in the proteome of senescing leaves, using protein MS data obtained from the same leaf groups used for imaging. Arabidopsis thaliana mature leaves were grouped according to their chlorophyll content: Dark Green (DG), Green (G), Light Green (LG) and Yellow (Y), containing 100, 45, 25 and 6.5% chlorophyll relative to DG, respectivelyArabidopsis thaliana mature leaves were grouped according to their chlorophyll content: Dark Green (DG), Green (G), Light Green (LG) and Yellow (Y), containing 100, 45, 25 and 6.5% chlorophyll relative to DG, respectively
Project description:RNA-sequencing was performed using Col-0 and hfr1-4pil1-2 seedlings upon 96 hours (96hr) low R:FR treatment. We analysed global gene expression changes in Arabidopsis thaliana in response to 96hr low R:FR, in order to identify the different effects of negative regulator in long-term treatments.
Project description:The two-spotted spider mite, Tetranychus urticae, is one of the most significant mite pests in agriculture that can feed on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. In order to refine the involvement of jasmonic acid (JA) in mite-induced responses, we analyzed transcriptional changes in tomato JA signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. We used microarray to assess global gene expression in Solanum lycopersicum def-1 cv. Castlemart upon jasmonic acid treatment and Tetranychus urticae attack.
Project description:Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remain scarce. Here the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles that are produced upon wounding, herbivory or infection with pathogens is studied. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal but not by defense hormones. In three independent biological experiments, Arabidopsis plants, ecotype Columbia (Col-0), were exposed either to 3 µM aerial E-2-hexenal or to the carrier MeOH for the mock treatment and rosette leaves were harvested after 1h, 3h and 24h.