Project description:The evaluation of mycotoxicity of type B trichothecenes using a yeast gene expression comparison analysis. The yeast BY4743 derivative PDR5 mutant was used for this study. The yeast cells were treated with trichothecene mycotoxins, and incubated at 25 degree for two hours, respectively. Total RNA was isolated with commercial kit (FastRNA Pro Red kit, Q-Biogene), and amplified RNA (aRNA) was synthesized with 3'IVT Express kit (Affymetrix). All samples were hybridized with Gene Chip (Yeast Genome 2.0 Array, Affymetrix), then each array chip was scanned by GeneChip Sanner 3000 (Affymetrix).
Project description:The evaluation of zymolyase treatment using yeast gene expression comparison analysis. The yeast BY4743 strain was used for this study. Yeast cells were treated with Zymolyase (final conc. 300 U/ml), and incubated at 37 degree for ten minutes. Digestion process was not provided for control sample. Total RNA was isolated with commercial kit (FastRNA Pro Red kit, Q-Biogene), and amplified RNA (aRNA) was synthesized with 3'IVT Express kit (Affymetrix). All samples were hybridized with Gene Chip (Yeast Genome 2.0 Array, Affymetrix), then each array chip was scanned by GeneChip Sanner 3000 (Affymetrix).
Project description:The Zap1 transcription factor is a central player in the response of yeast to changes in zinc status. Zap1 acts as both a positive and negative regulator of its target genes. We previously used transcriptome profiling with DNA microarrays to identify 46 potential Zap1 target genes in the yeast genome. In this study, we have used complementary methods to identify additional Zap1 target genes. With alternative growth conditions for the microarray experiments and a more sensitive motif identification algorithm, we have identified 31 new potential targets of Zap1 activation. Keywords: Yeast Saccharomyces cerevisiae whole genome array
Project description:We have developed a microarray intended for use in finding all transposons in a region of interest. By selectively amplifying and hybridizing transposon flanking DNA to our array, we can localize all transposons in the region present on our TIP-chip, a dense tiling array. We have tested our technology in yeast and have been successful. Keywords: transposon insertion profiling, genomic DNA, yeast
Project description:In order to establish the optimal conditions to study expression of genes of S. cerevisiae upon interaction with dendritic cells, Saccaromyces cerevisiae cells were cultured in presence of dendritic cells for 4 hours.<br>Dendritic cells were then treated with zymolase, the yeast cells recovered and lysed. RNA from lysed yeast cells was used for a transcription profiling analysis on Agilent arrays comparing internalized yeast with control yeast.