Dataset Information


Transcription profiling by array of hematopoietic stem cells from mice heterozygous for CREB binding protein

ABSTRACT: Myelodysplastic syndrome (MDS) is considered a disease of hematopoietic stem cell (HSC) origin. To begin to unravel the molecular mechanisms underlying the deregulation of HSCs in MDS, we performed comparative gene expression profiling on Crebbp+/- and wild type HSCs. We chose to isolate HSCs from the fetal liver (FLHSC) because at this stage there were no differences in cell number between Crebbp+/- and wild type fetal livers, suggesting no overt hematopoietic differences. Thus, any change in gene expression found in Crebbp+/- FLHSCs is likely to reflect the initially compromised genetic program of HSC regulation, as opposed to that of Crebbp+/- HSCs in adult bone marrow, where secondary changes in gene expression may also occur as compensatory mechanisms for a compromised or failing hematopoietic system. We used day 14.5 post coitus FLHSC (Sca-1+,Lin-,AA4.1+,c-Kit++) from wild type (wt) and Crebbp heterozygous (ht) embryos to examine changes in gene expression before overt myelodysplastic disease manifestation. Total RNA from wt and Crebbp+/- FLHSCs was isolated, PCR-amplified using the Ovation RNA amplification system and hybridized to Affymetrix Mouse 430 2.0 expression microarrays.



ORGANISM(S): Mus musculus  

PROVIDER: E-GEOD-18061 | ExpressionAtlas | 2014-07-03

REPOSITORIES: ExpressionAtlas

Dataset's files

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E-GEOD-18061-analysis-methods.tsv Tabular
E-GEOD-18061-atlasExperimentSummary.Rdata Rdata
E-GEOD-18061-configuration.xml Xml
E-GEOD-18061-percentile-ranks.tsv Tabular
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Mice heterozygous for CREB binding protein are hypersensitive to γ-radiation and invariably develop myelodysplastic/myeloproliferative neoplasm.

Zimmer Stephanie N SN   Lemieux Madeleine E ME   Karia Bijal P BP   Day Claudia C   Zhou Ting T   Zhou Qing Q   Kung Andrew L AL   Suresh Uthra U   Chen Yidong Y   Kinney Marsha C MC   Bishop Alexander J R AJ   Rebel Vivienne I VI  

Experimental hematology 20111220 4

Myelodysplastic syndrome is a complex family of preleukemic diseases in which hematopoietic stem cell defects lead to abnormal differentiation in one or more blood lineages. Disease progression is associated with increasing genomic instability and a large proportion of patients go on to develop acute myeloid leukemia. Primarily a disease of the elderly, it can also develop after chemotherapy. We have previously reported that CREB binding protein (Crebbp) heterozygous mice have an increased incid  ...[more]

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