Project description:With our experiments we have shown LP(Lactobacillus plantarum) mediated modification of immunogenic bone marrow derived dendritic cells (BMDCs) to acquire tolerogenic phenotype, to unravel the molecular mechanism of LP mediated immunomodulation we performed global transcriptomic Gut of old mice was reconstituted with LP, and RNA was extracted from LPS stimulated BMDCs from young, young treated with antibiotic cocktail, old and old-LP groups for gene array profiling by Agilent GeneChips.
Project description:Previous articles have reported that mouse cell lines infected with hepatitis virus do not produce interferon, which only causes natural immune responses when infected with macrophages and dendritic cells. Therefore, we collected the cells of MHV-infected mouse dendritic cells for 18 hours, and detected the immune-related factors and other altered genes in the cells after infection by Proteomics analysis
Project description:The goal of this experiment was to use global gene expression profiling to assess the global genetic reprogramming of spleen XCR1+ DC early after MCMV infection in vivo, using on Affymetrix Mouse Gene 1.0 ST Array. Comparison of the gene expression programs of XCR1+ dendritic cells isolated from the spleen of untreated versus d1.5 MCMV-infected wild-type C57BL/6 mice.
Project description:Investigation of the role of miR-29 on transcriptional responses to Mycobacterium tuberculosis (MTB) infection in human dendritic cells using gain- and loss-of-function approaches and microarray expression profiling. We assessed the impact of miR-29 perturbation on mRNA expression levels before and after MTB infection in dendritic cells using cells collected from 4 unrelated individuals. Cells were transfected using either a miR-29a mimic or miR-29 family inhibitor before infection with H37Rv for 24 hours. Gene expression was profiled by array and 4 technical replicates were performed to confirm reproducibility.
Project description:Gene expression changes in human dendritic cells after contact with either 10e4 or 10e7 CFU of Lactobacillus rhamnosus Lcr35 Bacterial cells were incubated for 3 hours with dendritic cells. Total RNA was extracted, quantified, and labeled with Cy3 or Cy5 (control, dendritic cells without bacteria). Hybridization was performed using Agilent Whole Human Genome microarray 4x44K (Agilent).