Project description:We prepared FLAG-tag knock-in mice for testis-specific polypeptides or transiently transfected HEK293T cells. Using these materials, we then explored the binding proteins to testis-specific polypeptides.

2021-07-06 | PXD027126 | JPOST Repository

E-MEXP-1040

Project description:Transcription profiling by array of testis from Fox3a knock-out mice

2014-07-09 | E-MEXP-1040 | ExpressionAtlas

Gene expression in wild type and Cagal102 knockout Candida albicans strains.

Project description:CaGAL102 is a sequence homolog of Rmlb. In Candida knock out of this gene causes abnormal hyphal morphogenesis and increased sensitivity to cell wall damaging agents. The knock out strain is also avirulent in mouse model of systemic infection. To get a larger insight into the function of the protein product of this gene we carried out global transcription analysis through micro array experiment. The gene is expressed under normal growth conditions and the knock out causes the cells to become hyphal under these conditions. Many of the cell wall proteins were upregulated recapitulating the cell morphology. Keywords: Candida albicans, Gene knockout, genome wide transcription profiling study

2011-03-15 | GSE20883 | GEO

Escherichia coli

Project description:anaerobic knock-out

| PRJNA90253 | ENA

Escherichia coli

Project description:aerobic knock-out

| PRJNA90251 | ENA

Saccharomyces cerevisiae

Project description:Effect of adk1 knock-out mutation on global transcription

| PRJNA103369 | ENA

Double-stranded RNA in testis

Project description:We precipitated dsRNA using the monoclonal J2 antibody and deep-sequenced the enriched samples from testis of juvenile dicer knock-out mice, age matched controls and adult animals. The dsRNA transcriptome is significantly less complex in juvenile mice as compared to adult controls and, possibly as a consequence, the knock-out of Dicer had only a minor effect of the total number of transcribed regions associated with dsRNA. The genes that potentially generate dsRNA are significantly expressed in isolated sperm cells with particular enrichment in pachytene spermatocytes.

2021-06-01 | GSE149814 | GEO

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