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Omics score: 4

Transcription profiling by array of transgenic rice plants expressing an isopentenyltransferase gene driven by PSARK under water stress

ABSTRACT: Transgenic rice plants expressing isopentenyltransferase (IPT), an enzyme that catalyzes the rate-limiting step in CK synthesis under the control of SARK, a maturation- and stress-inducible promoter. Increased CK production resulted in sink source alteration and enhanced drought tolerance of the transgenic plants. Flag leaf samples from well-watered (control) and water-stressed (3 days of withholding water) plants were collected for RNA extraction and hybridization on Affymetrix rice microarrays chip.

OTHER RELATED OMICS DATASETS IN: PRJNA131635

PROVIDER: E-GEOD-23211 | ExpressionAtlas | 2016-02-16

REPOSITORIES: ExpressionAtlas

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Cytokinin-mediated source/sink modifications improve drought tolerance and increase grain yield in rice under water-stress.

Peleg Zvi Z Reguera Maria M Tumimbang Ellen E Walia Harkamal H Blumwald Eduardo E

Plant biotechnology journal 20110201 7

Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isopentenyltransferase (IPT) gene driven by P(SARK) , a stress- and maturation-induced promoter. Plants were tested for drought tolerance at two yield-sensitive developmental stages: pr ...[more]

PMID: 21284800

Similar Datasets

Expression data from PSARK::IPT and wildtype rice plants

Project description:Transgenic rice plants expressing isopentenyltransferase (IPT), an enzyme that catalyzes the rate-limiting step in CK synthesis under the control of SARK, a maturation- and stress-inducible promoter. Increased CK production resulted in sink source alteration and enhanced drought tolerance of the transgenic plants. Flag leaf samples from well-watered (control) and water-stressed (3 days of withholding water) plants were collected for RNA extraction and hybridization on Affymetrix rice microarrays chip.

2011-10-26 | E-GEOD-23211 | biostudies-arrayexpress

Expression data from PSARK::IPT and wildtype rice plants

2011-10-27 | GSE23211 | GEO

Expression data from PSARK::IPT Nicotiana and wildtype plants

Project description:Stress-induced leaf senescence could be delayed in transgenic plants expressing isopentenyltransferase (IPT), an enzyme that catalyzes the rate-limiting step in CK synthesis under the control of SARK, a maturation- and stress-inducible promoter (Rivero et al., 2007). Increased CK production resulted in enhanced drought tolerance of the transgenic PSARK::IPT plants, with minimal yield loss (Rivero et al., 2007). We have used a large-scale expression profiling approach to investigate the effects of PSARK::IPT on gene expression to elucidate the molecular events associated with the CK-induced drought tolerance displayed by the PSARK::IPT plants. We used tomato genechips to capture the differential transcriptional responses of WT and PSARK::IPT plants under control and water stress conditions Leaf samples from well watered (1000 mL per day) and water stressed (300 mL per day) plants were collected for RNA extraction and hybridization on Affymetrix microarrays after 45 days of stress.

2010-01-09 | E-GEOD-19787 | biostudies-arrayexpress

Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice

Project description:Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes. Rice (Oryza sativa L.), a non-accumulator of glycinebetaine (GB), is highly susceptible to abiotic stress. Transgenic rice with chloroplast-targeted choline oxidase encoded by the codA gene from Arthrobacter globiformis has been evaluated for inheritance of transgene up to R5 generation and water-stress tolerance. During seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem II and they show better physiological performance, e.g. enhanced detoxification of reactive oxygen species compared to wild-type plants under water-stress. Survival rate and agronomic performance of transgenic plants is also better than wild-type following prolonged water-stress. Choline oxidase converts choline into glycinebetaine and H2O2 in a single step. It is possible that H2O2 /GB might activate stress response pathways and prepare transgenic plants to mitigate stress. To check this possibility, microarray-based transcriptome analysis of transgenic rice has been done. It unraveled altered expression of many genes involved in stress responses, signal transduction, gene regulation, hormone signaling and cellular metabolism. Overall, 165 genes show more than 2 folds up-regulation at P value <0.01 in transgenic rice. Out of these, at least 50 genes are known to be involved in plant stress response. Exogenous application of H2O2 or GB to wild-type plants also induces such genes. Our data show that metabolic engineering for GB is a promising strategy for introducing stress tolerance in crop plants and which could be imparted, in part, by H2O2- and/or GB-induced stress response genes.

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Expression data from PSARK::IPT Nicotiana and wildtype plants

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Expression data from leaves of rice plants overexpressing the OsCPK4 gene and control plants(empty vector) in normal conditions

Project description:The OsCPK4 gene is a member of the complex gene family of the Calcium-dependent protein kinases (CPKs) in rice. Expression of OsCPK4 is induced by high salinity, drought and the phytohormone abscisic acid. The OsCPK4 protein localizes to the plasma membrane. Transgenic rice overexpressing OsCPK4 enhances tolerance to salt and drought stress, the transgenic plants having stronger water-holding capability than control plants. Microarray analysis of OsCPK4 rice plants revealed up-regulation of genes involved in metabolism, particularly lipid metabolism, as well as genes involved in oxidative stress and redox control. Meanwhile, OsCPK4 overexpression has no impact on the expression of the well-characterized abiotic stress-associated transcription factors (i.e. DREB and NAC), or the typical salt and drought-inducible genes (i.e. LEA genes, including Dehydrin genes). Under salt stress conditions, the OsCPK4 transgenic lines showed lesser membrane lipid peroxidation as compared to control plants, indicating that OsCPK4 rice plants have a better capacity to prevent oxidative damage in cellular membrane lipids. Collectively, our data suggest that OsCPK4-mediated processes protect the plant cell from uncontrolled redox reactions affecting membrane functions, which, in turn, results in salt and drought tolerance. OsCPK4 shows great promise for genetic improvement of tolerance to abiotic stress in rice.

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Transcription profiling of rice indica after glycinebetaine-induced water-stress tolerance in codA-expressing transgenics

2009-06-24 | E-GEOD-15448 | biostudies-arrayexpress

Expression analysis of CaMsrB2-transformed rice (Oryza sativa L. cv. ILMI) without/with Bar selection marker under drought stress conditions

Project description:Methionine sulfoxide reductases catalyze the reduction of MetSO back to the correct Met residue. Previously, the gene of Capsicum annuum methionine sulfoxide reductase B2 was isolated and CaMSRB2-overexpressing tomato shows enhanced growth, which may trigger increased resistance to the pathogens. To assess the role of this enzyme in rice, we generated transgenic lines under the control of the rice Rab21 (responsive to ABA protein 21) promoter with/without Bar marker gene. Several physiological tests such as MV and Fv/Fm, indicators of an oxidative stress-inducing agent and a potential maximal PSII quantum yield, respectively strongly suggested CaMSRB2 confers drought tolerance to rice. Using 3M-bM-^@M-2-tiling microarray covering the whole rice genes, we carried out genome-wide expression analyses with CaMsrB2-transformed rice (Oryza sativa L. cv. ILMI). Rice was grown in port for six weeks and treated with drought by water withholding for two days. A total of 15 chips were used for the microarray experiment. RNA was extracted from plants just before and 2 days after the duration of water withdrawal for the control and the comparison, respectively. Experiments were performed with three or two biological replicates.

2013-04-01 | E-GEOD-41204 | biostudies-arrayexpress

Gene expression data of OsHMGB707 overexpression and RNAi transgenic rice plants under normal conditions

Project description:High mobility group (HMG) proteins play an important role in regulation of gene transcription through modulate the structure of DNA. In this study, OsHMGB707, a HMG gene localized in rice drought resistance QTL interval, was isolated and the function on rice stress resistance was identified. Overexpression of OsHMGB707 significantly enhanced the drought resistance of the transgenic rice plants, whereas the OsHMGB707-RNAi transgenic rice plants exhibited slightly decrease in drought stress tolerance. To search the downstream genes regulated by OsHMGB707, we performed microarray analysis of the OsHMGB707-overexpressing, OsHMGB707-RNAi and wild-type plants under both normal conditions using Affymetrix Rice Genome Genechip. 21-day-old plants of the OsHMGB707-overexpressing line OE1, OsHMGB707-RNAi line RNAi1 as well as the wild-type plants were used in the normal condition.

2019-05-08 | GSE130821 | GEO

Transcription profiling of rice plants over-expressing OsNAC5

Project description:Gene expression profiles in transgenic rice plants that overexpressed OsNAC5, driven by the maize ubiquitin promoter (UBIpro::OsNAC5).

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