Project description:We report the transcriptomic response of a moderately growing subisolate from the culture of Botryococcus sp. CCALA-779 upon nitrogen deprivation (ND). Examination of transcriptomes upon nitrogen deprivation (ND). B.braunii_non_redundant_transcriptome_assembly_61220.fasta: non-redundant assembly (with 61220 in number) of B.braunii 779 transcriptome in this study B.braunii_annotated_12292.fasta: investigated ESTs (with 12292 in number, which perfectly matches with our processed RNA expression profiling) in this study
Project description:We report the transcriptomic response of a moderately growing subisolate from the culture of Botryococcus sp. CCALA-779 upon nitrogen deprivation (ND).
Project description:In order to identify possible RNAs regulated by small RNA NsiR3 we have analyzed the responses to nitrogen removal in a mutant lacking nsiR3 (ΔnsiR3). RNA samples were isolated from cells growing in the presence of combined nitrogen (ammonium) or after 8 h of nitrogen deprivation.
Project description:Varying levels of nitrogen deprivation can improve lipid accumulation differently in microalgae, whose underlying regulations are unknown. To investigate the related mechanisms, Auxenochlorella pyrenoidosa was exposed to 1.5, 1, 0.5 and 0 g/L sodium nitrate (NaNO3) for 5 days and RNA-seq were detected.
Project description:The budding yeast S. cerevisiae responds to depletion of iron in the environment by activating Aft1p, the major iron-dependent transcription factor, and by transcribing systems involved in the uptake of iron. Here we have studied the transcriptional response to iron deprivation, and have identified new Aft1p target genes. We find that other metabolic pathways are regulated by iron: biotin uptake and biosynthesis, nitrogen assimilation, and purine biosynthesis. Two enzymes active in these pathways, biotin synthase and glutamate synthase, require an iron-sulfur cluster for activity. Iron deprivation activates transcription of the biotin importer and simultaneously represses transcription of the entire biotin biosynthetic pathway. Multiple genes involved in nitrogen assimilation and amino acid metabolism are induced by iron deprivation, while glutamate synthase, a key enzyme in nitrogen assimilation, is repressed. A CGG palindrome within the promoter of glutamate synthase confers iron-regulated expression, suggesting control by a transcription factor of the binuclear zinc cluster family. We provide evidence that yeast subjected to iron deprivation undergo a transcriptional remodeling, resulting in a shift from iron-dependent to parallel, but iron-independent, metabolic pathways. A dose response design type examines the relationship between the size of the administered dose and the extent of the response of the organism(s). Keywords: dose_response_design
Project description:Aligned whole-genome sequencing and RNA-seq of localised prostate cancer for study 'Loss of SNAI2 in prostate cancer correlates with clinical response to androgen deprivation therapy'.