Genomics

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Transcription profiling by array of Arabidopsis after treatment with glycerol-3-phosphate


ABSTRACT: Goal of this study was to determine changes in transcription profile in mock versus G3P treated plants. Arabidopsis (Col-0 ecotype) plants were infiltrated with petiole exudate, with or without G3P, and distal leaves were sampled 24 h post treatments. Keywords: Glycerol-3-Phosphate, Exudate, Salicylic acid, Arabidopsis, Systemic Acquired Resistance, Defense 3 mock-treated and 3 G3P-treated biological replicates were analyzed (one array each) Total RNA was isolated from four-week-old plants using TRIZOL method. The experiment was carried out in triplicate and a separate group of plants was used for each set. RNA was processed and hybridized to the Affymetric Arabidopsis ATH1 genome array GeneChip following the manufacturers instructions (http://www.affymetrix.com/Auth/ support/downloads/manuals/expression_analysis_technical_manual.pdf ). All probe sets on the Genechips were assigned hybridization signal above background using Affymetrix Expression Console Software v1.0 (http://www.affymetrix.com/Auth/support/downloads/manuals /expression_console_userguide.pdf ). Data was analyzed using the nonparametric Wilcoxen Rank Sum test. Fold changes are reported as ratios of medians. The P values were calculated individually for each probe set.

REANALYSIS of: E-GEOD-26973

OTHER RELATED OMICS DATASETS IN: PRJNA135915

ORGANISM(S): Arabidopsis thaliana  

PROVIDER: E-GEOD-26973 | ExpressionAtlas | 2014-07-03

REPOSITORIES: ExpressionAtlas

Dataset's files

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Action DRS
E-GEOD-26973-analysis-methods.tsv Tabular
E-GEOD-26973-atlasExperimentSummary.Rdata Rdata
E-GEOD-26973-configuration.xml Xml
E-GEOD-26973-percentile-ranks.tsv Tabular
E-GEOD-26973-zoomifications-log.tsv Tabular
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Publications


Glycerol-3-phosphate (G3P) is an important metabolite that contributes to the growth and disease-related physiologies of prokaryotes, plants, animals and humans alike. Here we show that G3P serves as the inducer of an important form of broad-spectrum immunity in plants, termed systemic acquired resistance (SAR). SAR is induced upon primary infection and protects distal tissues from secondary infections. Genetic mutants defective in G3P biosynthesis cannot induce SAR but can be rescued when G3P i  ...[more]

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