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E-GEOD-28227

ABSTRACT: Comparison of gene expression profiles between erf5/6 and WT Arabidopsis in response to chitooctaose

PROVIDER: E-GEOD-28227 | ExpressionAtlas | 2015-05-22

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-28227-analysis-methods.tsv	Tabular
	E-GEOD-28227-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-28227-configuration.xml	Xml
	E-GEOD-28227-percentile-ranks.tsv	Tabular
	E-GEOD-28227.condensed-sdrf.tsv	Tabular

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Project description:Transcription factors ERF5 and ERF6 may play a regulatory role in regulating the expression of chitin-responsive genes. We compared the gene expression profiles between the double mutant erf5/6 and WT plants in response to chitooctaose using the Affymetrix Arabidopsis Whole Genome Arrays. Both the double mutant erf5/6 and WT plants were grown in liquid MS medium in a growth chamber with constant light. Two-week-old seedlings were treated with chitooctaose at a final concentration of 1 uM for 30 minutes. The controls were similarly treated with an equivalent amount of ddH2O. The treated seedlings were harvested for RNA isolation. Three biological replicates were conducted for the experiment.

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