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E-GEOD-30531

ABSTRACT: Expression data of A375 melanoma cells after DMSO or MLN4924 treatment from 1 hour to 24 hour

OTHER RELATED OMICS DATASETS IN: PRJNA143311

PROVIDER: E-GEOD-30531 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-30531-analysis-methods.tsv	Tabular
	E-GEOD-30531-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-30531-configuration.xml	Xml
	E-GEOD-30531-percentile-ranks.tsv	Tabular
	E-GEOD-30531.condensed-sdrf.tsv	Tabular

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Project description:Expression data of A375 melanoma cells after DMSO or MLN4924 treatment from 1 hour to 24 hour

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Synergy from Gene Expression and Network Mining (SynGeNet) method predicts genotype-specific synergistic drug combinations in melanoma

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E-GEOD-39091

Project description:Expression data of A375 melanoma cells after DMSO or MLN4924 +/- Nutlin treatment for 26 hours

2025-01-24 | E-GEOD-39091 | ExpressionAtlas

Expression data from BRAFV600E A375 melanoma cells treated with vehicle or vemurafenib

Project description:Vemurafenib is a BRAF inhibitor with specificity for the most common BRAF mutant encountered in melanomas (BRAFV600E). Vemurafenib suppresses the proliferation of BRAF mutant human melanoma cells by suppressing downstream activation of the MEK/ERK mitogen activated protein kinases. We used microarrays to examine the transcriptional response of a vemurafenib-sensitive BRAFV600E human melanoma cell line (A375) to vemurafenib in order to further delineate the mechanisms by which BRAFV600E drives cell proliferation and energy metabolism in human melanoma. BRAFV600E A375 human melanoma cells were treated with vehicle (0.1% DMSO) or 10 uM vemurafenib for 24 h after which total RNA was extracted. Cells were prepared and RNA was extracted in 3 separate batches (three different cell stocks on three separate days) providing three independent replicates (n=3). Paired replicates (prepared from the same stock of cells on the same day) are denoted by A, B and C.

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Analysis of A375 and A375 clones that acquired resistance to GSK2118436 after treatment with GSK2118436 (GSK436), GSK1120212 (GSK212), or the combination of GSK2118436 and GSK1120212 for 24 hour

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Project description:Unsupervised hierarchical clustering revealed a strong similarity in gene modulation resulting from either compound treatment or BRAF ablation mediated by RNA interference relative to DMSO-treated control samples . Experiment Overall Design: We have generated melanoma A375 cells stably expressing a shRNA construct for doxycycline-inducible knockdown of B-Raf (2 mg/ml Dox, 48h). Small molecule treatment was at 1000nM R341787, 24h.

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