E-GEOD-32322
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA147271
PROVIDER: E-GEOD-32322 | ExpressionAtlas | 2014-07-03
REPOSITORIES: ExpressionAtlas
Similar Datasets
Project description:Transcriptional profiling with next-generation sequencing methods refined our understanding of the N. crassa transcriptional response to cellulose and demonstrated that the newly characterized transcription factors clr-1 and clr-2 were required for the bulk of that response including induction all major cellulase and some major hemicellulase genes. N. crassa pregrown in Sucrose and transferred to Avicel (cellulose), Sucrose or media with no carbon added. Biological triplicates used to identify differentially expressed genes in WT. Single libraries for mutant strains identify which genes show deficient regulation in response to Avicel. Note: Samples named "cdr1" and "cdr2" correspond to the genes clr-1 and clr-2 respectively.
2012-04-23 | E-GEOD-35227 | biostudies-arrayexpress
Project description:Transcription array by profiling in WT and SRC-2 null mouse liver
| PRJNA238224 | ENA
Project description:Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge into gene expression under nutrients depletion and senescence, however, that of monocot model plant rice under sucrose depletion is still under investigation. Here, the time-course monitoring of gene expression profiles in sucrose-starved rice (Oryza sativa cv Tainung67) suspension cells was investigated by 21495 probes-containing Agilent rice chip. In sucrose-starved cells, the induced vacuolar biogenesis was coincided with the significantly upregulated expression of genes encoding H+-pyrophosphatase, ï?¤-TIP, one putative ï?¡-TIP, several vacuolar proteases and proteinase inhibitors, and one OsATG3. To survey the overall metabolic adaptations under sucrose depletion the genes significantly alternating expression level were incorporated into multiple metabolic pathways. The majority of genes encoding enzymes involved in biosynthesis and degradation pathways of various macromolecules were comprehensively down- and upregulated, respectively, by sucrose starvation. Transcriptional regulation of gene expression is important for the physiological adaptations to environmental stress and many transcription factors, including bZIPs, NACs, and WRKY showed significant increase in transcript levels under sucrose starvation. Concurrently, statistical analysis reveals that their corresponding consensus cis-elements, such as ABA-responsive element, CACG, ACI, ACII and CTTATCC, are frequently found in the promoter regions of many Suc starvation-upregulated genes. Particle bombardment-mediated transient promoter activity assays further showed that the CTTATCC, derived form TATCCA, and the AC motifs, are the promising sucrose starvation responsive activators in sucrose-starved rice suspension cells. Experiment Overall Design: We used Agilent rice gene chips to investigate the gene expression profiling in rice cells after periods of 12, 24 and 48 h Suc starvation. To eliminate the effect of osmotic stress on alternation of gene expression, an additional set of control, 88 mM Sorbitol in MS medium verse Suc-free samples for 12 h Suc starvation, was also applied for array analysis.
2008-06-14 | E-GEOD-5853 | biostudies-arrayexpress
Project description:Transcription profiling by array of adult abdominal/thorasic fat body or midgut in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.
2014-08-18 | E-MTAB-1020 | biostudies-arrayexpress
Project description:Transcriptomic profiling using Drosophila heads reveals early gene expression responses to transient paraquat exposure. We performed RNAseq profiling of heads from the wild type (WT) Drosophila strain, Canton S (males only), that were fed either 2.5% sucrose (control) or 5 mM PQ in 2.5% sucrose for 12 h.
2019-09-16 | GSE135919 | GEO
Project description:As no commercial array is available for sorghum microarray analysis, we designed an array based on the annotation of Sbi1.4 gene set and the available 209,835 sorghum ESTs from the NCBI EST database. The array will be used for investigating the expression divergence between grain and sweet sorghum lines under normal and sucrose treatments
2012-04-08 | GSE36689 | GEO
Project description:To investigate whether the transcriptional response to carbon (C) depletion and sucrose re-addition depend on the duration of C-depletion, Arabidopsis thaliana seedlings growing in liquid culture in weak continuous light were harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium, and 30 min after resupplying sucrose at each of these times. After removing sucrose, soluble sugars fell strongly within 3 h, and starch was gradually depleted over 24 h, and hexose phosphates and ATP declined gradually over 72 h. Expression profiling using ATH arrays pointed to Overall the transcriptional response pointed to early transcriptional remodelling of metabolism to conserve C, followed by induction of photosynthesis and pathways that recycle C, and repression of growth-related processes. The time-dependent transcriptional response to C-depletion differed from that during a light/dark cycle and an extended night. Re-supplying sucrose for 30 min led to near-complete recovery of seedling sucrose levels, partial recovery of reducing sugars and phosphorylated intermediates, but no immediate change of starch or ATP. The rapid transcriptional response to sucrose readdition was conserved across the entire C-depletion time course, became larger with time. , and was highly enriched for regulatory genes. Whilst there was a rapid decrease of many C-depletion-induced transcripts, fewer transcripts increased. The majority of the transcripts that responded rapidly after resupplying sucrose also decreased after treating C-depleted seedlings with the transcriptional inhibitor cordycepin A, pointing to an important role for transcript turnover in the rapid response to sucrose.
2015-10-12 | E-MTAB-3143 | biostudies-arrayexpress