E-GEOD-32893
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA146633
PROVIDER: E-GEOD-32893 | ExpressionAtlas | 2015-09-21
REPOSITORIES: ExpressionAtlas
Similar Datasets
Project description:Human bronchial epithelial cells exposed to A. alternata spores
| PRJNA146633 | ENA
Project description:Human bronchial epithelial cells exposed to interleukin 1 beta
| PRJNA553230 | ENA
Project description:The goal was to assess global gene expression changes in primary human bronchial epithelial cells exposed to environmental tobacco smoke (ETS) condensate. ETS-C was standardized by HPLC analysis and two timepoints of exposure in two different donor bronchial epithelial cell populations were assessed. These findings demonstrate that even short exposure (4.5 h) to ETS is sufficient to induce a stress response, as reflected by decreased antioxidant levels, induced HSP family members, and modulation of the family of glutathione metabolism enzymes in primary human lung cells. Upon longer exposures (48 h) with ETS-condensate, bronchial epithelial cells arrest at the G2/M phase of the cell cycle. Taken together, these data support a stress-induced state in primary human bronchial epithelial cells that culminates in cell cycle arrest. Keywords: time course, comparative, stress response
2006-12-25 | GSE4234 | GEO
Project description:<h4><strong>BACKGROUND: </strong>Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine-protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development.</h4><h4><strong>METHODS: </strong>Calu-3 cells were cultivated in air-liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry.</h4><h4><strong>RESULTS: </strong>We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical-basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L-arginine, L-kynurenine and L-methionine.</h4><h4><strong>CONCLUSION: </strong>This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine-protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence.</h4>
2023-08-22 | MTBLS1755 | MetaboLights
Project description:Gene expression microarrays were used to compare gene alterations induced by exposure to equitoxic doses of crocidolite asbestos and cristobalite silica in an isolate of normal human bronchial epithelial cells. Primary human bronchial epithelial cells (NHBE) were exposed to crocidolite asbestos, cristobalite silica, and saline as control for 24 hours. Total RNA was extracted from cell cultures, 3 separate cultures were used for each condition. The isolated RNA was used to analyze mRNA expression via Affymetix GeneChip Human U133A 2.0 arrays.
2014-10-29 | E-GEOD-62769 | biostudies-arrayexpress
Project description:normal human bronchial epithelial cultures from two cultures in parallel exposed to cigarette smoke (CS) or air (mock) at timepoints 4 hours and 24 hours. Keywords = cigarette smoke Keywords = microarray Keywords = bronchial cell Keywords = tobacco Keywords: time-course
2005-02-23 | GSE2302 | GEO
Project description:This present study is the first to investigate the global changes in host gene expression during the interaction of human bronchial epithelial cells and live Alternaria spores. Human bronchial epithelial cells (BEAS2-B) were exposed to spores or media alone for 24 hours. RNA was collected from three biological replicates/treatment and used to assess changes in gene expression patterns using Affymetrix Human Genome U133 Plus 2.0 Arrays. Interestingly, many cytokine/chemokine immune response genes were upregulated. Genes involved in cell death, retinoic acid signaling, TLR3, and interferon response pathways were also significantly upregulated.
2011-12-01 | GSE32893 | GEO
Project description:This study is the first to show transfer of regulatory bacterial sRNAs from bacterial OMVs to host cells. Demonstration of transfer of bacterial sRNA from Pseudomonas aeruginosa OMVs to host cells in two cell types. RNA isolated from PA14 OMV exposed primary human bronchial epithelial cells or CFBE41o- bronchial epithelial cells as well as unexposed control cells was analyzed by small RNA-Seq. Primary cell exposures were performed on two donors and exposures of CFBE41o- cells were done in triplicate.
2016-05-13 | E-GEOD-80421 | biostudies-arrayexpress
Project description:Organotypic culture of human primary bronchial epithelial cells is a useful in vitro system to study normal biological processes and lung disease mechanisms, to develop new therapies, and to assess the biological perturbations induced by environmental pollutants. Herein, we further develop this in vitro model as a standard human airway assay by comparing the biological response observed after cigarette smoke (CS) exposure in vitro and published data from human bronchial epithelium of smokers. To this end, we exposed differentiated normal human bronchial epithelial cells (AIR-100 tissue) to mainstream CS for 7, 14, 21, or 28 min at the air-liquid interface and investigated various biological endpoints (e.g., gene expression and microRNA profiles, MMP-1 release) at multiple post-exposure time points (0.5, 2, 4, 24, 48 hours).
2012-04-01 | E-MTAB-874 | biostudies-arrayexpress
Project description:Organotypic culture of human primary bronchial epithelial cells is a useful in vitro system to study normal biological processes and lung disease mechanisms, to develop new therapies, and to assess the biological perturbations induced by environmental pollutants. Herein, we further develop this in vitro model as a standard human airway assay by comparing the biological response observed after cigarette smoke (CS) exposure in vitro and published data from human bronchial epithelium of smokers. To this end, we exposed differentiated normal human bronchial epithelial cells (AIR-100 tissue) to mainstream CS for 7, 14, 21, or 28 min at the air-liquid interface and investigated various biological endpoints (e.g., gene expression and microRNA profiles, MMP-1 release) at multiple post-exposure time points (0.5, 2, 4, 24, 48 hours).
2012-04-01 | E-MTAB-877 | biostudies-arrayexpress