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E-GEOD-35258

ABSTRACT: Comparison of low water potential (drought)-regulated gene expression in wild type (Col-0) and the hai1-2 (At5g59220) mutant

OTHER RELATED OMICS DATASETS IN: PRJNA152829

PROVIDER: E-GEOD-35258 | ExpressionAtlas | 2015-06-01

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-35258-analysis-methods.tsv	Tabular
	E-GEOD-35258-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-35258-configuration.xml	Xml
	E-GEOD-35258-percentile-ranks.tsv	Tabular
	E-GEOD-35258.condensed-sdrf.tsv	Tabular

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Arabidopsis thaliana

Project description:Comparison of low water potential (drought)-regulated gene expression in wild type (Col-0) and the hai1-2 (At5g59220) mutant

| PRJNA152829 | ENA

Comparison of low water potential (drought)-regulated gene expression in wild type (Col-0) and the hai1-2 (At5g59220) mutant

Project description:The Clade A PP2C Highly ABA-Induced1 (HAI1, At5g59220) is strongly up-regulated by low water potential in an ABA-dependent manner. Using knockout mutants of hai1, we found that HAI1 functions as a negative regulator of low water potential-induced proline and osmoregulatory solute accumulation. We also found a relatively weak and limited interaction of HAI1 with the RCAR/PYL family of ABA receptors. This, plus its induced expression, suggest that HAI1 remains active during stress and attenuates specific aspects of drought response. Microarray experiments were used to identify genes differentially expressed in hai1-2 (SALK_108282) versus wild type under both unstress control conditions and after low water potential (i.e., drought, water deficit). A relatively long term (96 h) low water potential treatment was used as phenotypes of hai1-2 were most apparent after this longer term low water potential treatment. Seven-day-old seedlings of wild type (Col-0) or hai1-2 were transferred from agar plates of control media to low water potential stress (PEG-infused agar plates at -1.2 MPa) or to fresh control media (-0.25 Mpa). Samples (whole seedlings) were collected 96 h after transfer. Three independent experiments were conducted with one sample of each treatment (wild type stress and control, hai1-2 stress and control) collected from each experiment.

2012-02-03 | E-GEOD-35258 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Comparison of low water potential (drought) regulated gene expression in wild type (Col-0) and egr1-1egr2-1 (At3g05640/At5g27930) mutant.

| PRJNA290646 | ENA

Expression data in an Arabidopsis cytokinin (CK)-signaling mutant, ahp2,3,5 and its wild type plant (Col-0) under drought and well-water (control) conditions.

Project description:To understand the role of CK-signaling components, AHPs (His-containing phosphotransfer proteins) in drought stress response, we have employed transcriptional profiling of ahp2,3,5 and it's wild type plant, Col-0 under drought and well-water (control) conditions. Aligent’s Whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.

2013-02-15 | GSE42290 | GEO

Control, low water potential (drought) stress and stress release transcriptome analysis of mutants impaired in proline (Delta1-pyrroline-5-carboxylate synthase1-4; p5cs1-4) or abscisic acid (aba2-1) synthesis.

Project description:mRNA sequencing was used to identify genes differentially expressed in Delta1-pyrroline-5-carboxylate synthase1-4 (p5cs1-4) mutants deficient in low water potential-induced proline accumulation and the Abscisic Acid (ABA) deficient mutant aba2-1 compared to Col-0 wild type. Three treatments were analyzed for each genotype: unstressed control, a 96 hour low water potential (-1.2 MPa) treatment and a stress-release treatment where seedlings were removed from the stress treatment and transferred back to control media for 10 hours. Approximately 100 genes were found to be significantly up-regulated in p5cs1-4 compared to wild type in both the control and stress treatments with an equal number down regulated. There was also a substantial transcription response to the stress release treatment which varied between wild type, p5cs1-4 and aba2-1.

2016-08-05 | GSE75933 | GEO

Constitutive expression of JASMONATE RESISTANT 1 elevates content of several jasmonates and primes Arabidopsis thaliana to better withstand drought

Project description:Purpose: In this study we investigated the role of JASMONATE RESISTANT 1 (JAR1) and JAR1 mediated JA-Ile formation in drought stress tolerance in Arabidopsis thaliana. Methods: Global transcriptional changes in a newly generated over-expression line (JAR1-OE; 35S::JAR1-1-YFP)), a T-DNA insertion line in the JAR1 locus (jar1-11;SALK_034543), and wild-type Col-0 were investigated by RNA-seq analyses of rosette leaves from 32 day-old plant that were either well-watered (control) or not watered after day 18 (drought). Plants were grown on soil under long-day conditions Results: Under control conditions, using a stringent cut-off (DESeq, adjusted to FDR < 0.01 and LogFC ≥ 1), we found only four differentially expressed genes (DEGs) between jar1-11 and Col-0, all of them downregulated. By contrast, we found 339 DEGs between JAR1-OE and Col-0, of which 134 were downregulated and 205 were upregulated. A comparison of the RNA-seq data from Col-0 between control and drought conditions revealed 3401 DEGs, of which 2023 were down- and 1378 upregulated. By comparison, jar1-11 plants, which were most heavily affected by drought stress, showed a much higher number (6139 in total; 2616 up- and 3523 down-regulated) of DEGs, while the more drought-tolerant JAR1-OE line displayed a lower number (2025 in total; 971 up- and 1054 down-regulated) of DEGs. 2411 DEGs were found between Col-0 and jar1-11 under drought among which 966 genes showed a higher and 1445 genes a lower expression level in jar1-11. On the other hand, out of 998 DEGs found between Col-0 and JAR1-OE under drought, 737 genes showed a higher and 261 genes a lower expression level in JAR1-OE. Moreover, we found 391 DEGs counter-regulated between jar1-11 and JAR1-OE. Conclusion:RNA-seq analysis and additional experiments of plants under control and drought stress conditions provided insight into the molecular reprogramming caused by the alteration in JA-Ile content.

2022-07-25 | GSE196602 | GEO

Arabidopsis hsi2 vs. Col-0 Drought Arrays

Project description:Transcriptional profiling of rossette leaves comparing wild type (Col-0) and the mutant (hsi2-5) under no drought or simulated drought stress.

2014-01-24 | GSE39347 | GEO

Comparison of low water potential (drought)-regulated gene expression in wild type (Col-0) and the hai1-2 (At5g59220) mutant

2012-02-03 | GSE35258 | GEO

Genome-wide expression analysis of Arabidopsis Col-0 plants under BTH treatment and high or low humidity

Project description:The goal of this study is to demonstrate the global expression profile of four-week-old Arabidopsis Col-0 wild type plant leaves in response to high or low air humidity, under water or BTH (SA analog) treatment.

2023-10-17 | GSE210893 | GEO

Transcriptome analysis using RNA sequencing conducted for wild type (Col-0) and ahl10-1 mutant under control conditions and after 96 h at moderate low water potential (-0.7 MPa)

Project description:RNA sequencing was used to identify genes differentially expressed (DEG) in ahl10-1 mutant compared to Col-0 wild type. Two treatments were analyzed for each genotype: unstressed control and a 96 hour low water potential (-0.7 MPa) treatment. P<0.05 and fold change ≥ 1.25 used as cut off value for DEG. Approximately 2000-3000 genes were found to be significantly up and down-regulated in the wild type stress versus control treatment using . In control treated seedlings, 10 or less than 10 genes are up and down-regulated in ahl10-1 mutant. In the stress treatment, ahl10-1 has 19 and 42 genes that are up and downregulated, respectively.

2018-07-20 | GSE112368 | GEO

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