Project description:The Xenopus POU class V transcription factor XOct-25 has been shown to inhibit BMP-dependent epidermal differentiation and promote neural induction in the ectoderm during early embryogenesis. In order to identify genes that act downstream of XOct-25, transcriptional profile of Xenopus ectodermal cells overexpressing XOct-25 was compared with control cells by using a DNA microarray method. Two independent experiments. Each experiment contains ectodermal cells overexpressing XOct-25 and corresponding control cells. Xenopus embryos were injected at the 8-cell stage with mRNA encoding GR-XOct-25, a hormone-inducible version of XOct-25. Explants from stage 9 embryos were treated with or without dexamethazone (DEX) until the sibling embryos reached stage 10.5, when they were used for RNA extraction. The explants cultured without DEX was used as a control sample. biological replicates: Sample name XOct-exp 1, Sample name XOct-exp 2
Project description:Proteome analysis of embryonic neural progenitor cells, comparing slowly-dividing and rapidly-dividing neural progenitor cells (NPCs). Expression of H2B-GFP was transiently induced in transgenic embryos with a Tet-on system by a single injection of 9TB-Dox at E9.5. Subsequently, NPCs (CD133+CD24– cells) were isolated from the LGE based on the GFP fluorescence intensity at E17.5. The top 10% of NPCs according to H2B-GFP fluorescence intensity (GFP++ cells) were collected as slowly dividing NPCs, whereas the middle 45% to 75% (GFP+ cells) and bottom 10% (GFP– cells) of NPCs were collected as rapidly dividing NPC fractions.