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E-GEOD-39196

ABSTRACT: Effect of Gadd45a overexpression on mouse skeletal muscle mRNA levels.

PROVIDER: E-GEOD-39196 | ExpressionAtlas | 2015-09-29

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-39196-analysis-methods.tsv	Tabular
	E-GEOD-39196-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-39196-configuration.xml	Xml
	E-GEOD-39196-percentile-ranks.tsv	Tabular
	E-GEOD-39196.condensed-sdrf.tsv	Tabular

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Mus musculus

Project description:Effect of Gadd45a overexpression on mouse skeletal muscle mRNA levels.

| PRJNA170209 | ENA

Effect of Gadd45a overexpression on mouse skeletal muscle mRNA levels.

2012-09-15 | GSE39196 | GEO

Effect of Gadd45a overexpression on mouse skeletal muscle mRNA levels.

Project description:Gadd45a is a stress-induced protein that causes skeletal muscle atrophy. The goal of these studies was to determine the effects of Gadd45a overexpression on mRNA levels in mouse skeletal muscle. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12, 2012. Tibialis anterior (TA) muscles from muscle-specfic ATF4 knockout mice (ATF4 mKO) were transfected with either 20 mg empty plasmid (pcDNA3) (left TA) or 20 mg pCMV-FLAG-Gadd45a (right TA) and harvested 7 days later. mRNA levels in Gadd45a-transfected muscles were normalized to levels in control transfected muscles.

2012-09-15 | E-GEOD-39196 | biostudies-arrayexpress

E-GEOD-39194

Project description:Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

2015-09-29 | E-GEOD-39194 | ExpressionAtlas

Mus musculus

Project description:Effect of ATF4 overexpression on mouse skeletal muscle mRNA levels

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E-GEOD-39009

Project description:Expression data from mouse skeletal muscle

2015-09-29 | E-GEOD-39009 | ExpressionAtlas

Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

2012-09-15 | GSE39194 | GEO

Effect of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes.

Project description:ATF4 is a bZIP transcription factor that that promotes skeletal muscle atrophy. The goal of these studies was to determine the effects of ATF4 overexpression on mRNA levels in differentiated C2C12 myotubes. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12,2012 C2C12 myotubes were infected with adenovirus co-expressing eGFP and ATF4-FLAG. Control myotubes were infected with adenovirus co-expressing eGFP and a transcriptionally inactive ATF4 construct (ATF4∆bZIP).

2012-09-15 | E-GEOD-39194 | biostudies-arrayexpress

Effect of denervation on mouse skeletal muscle mRNA levels.

Project description:Muscle denervation causes skeletal muscle atrophy. The goal of these studies was to determine the effects of denervation on skeletal muscle mRNA levels in C57BL/6 mice. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12, 2012.

2012-09-15 | GSE39195 | GEO

Gadd45a Promotes Skeletal Muscle Atrophy by Forming a Complex with the Protein Kinase MEKK4

Project description:Skeletal muscle atrophy is a serious and highly prevalent condition that remains poorly understood at the molecular level. Previous work found that skeletal muscle atrophy involves an increase in skeletal muscle Gadd45a expression, which is necessary and sufficient for skeletal muscle fiber atrophy. However, the direct mechanism by which Gadd45a promotes skeletal muscle atrophy was unknown. To address this question, we biochemically isolated skeletal muscle fiber proteins that associate with Gadd45a as it induces skeletal muscle atrophy in living mice. We found that Gadd45a interacts with multiple proteins in skeletal muscle fibers, including, most prominently, the MAP kinase kinase kinase MEKK4. Furthermore, by forming a complex with MEKK4 in skeletal muscle fibers, Gadd45a increases MEKK4 protein kinase activity, which is sufficient to induce skeletal muscle fiber atrophy and required for Gadd45a-mediated skeletal muscle fiber atrophy. Together, these results identify a direct biochemical mechanism by which Gadd45a induces skeletal muscle atrophy and provide new insight into way that skeletal muscle atrophy occurs at the molecular level.

2016-05-17 | MSV000079730 | MassIVE

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