Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of mRNA expression profile for colon tissues from wildtype and CARD9 knockout mice treated with AOM/DSS
Project description:We performed high-throughput RNA sequencing to characterize possible differences in the transcriptome of murine aortic Vascular Smooth Muscle cells from both 26 weeks-old ApoE knockout and ApoE/Neil3 double knockout mice fed a regular diet.
Project description:We report the application of sequencing technology for high-throughput profiling of RUNX1 transcription factor occupancy in mouse EML cells. RUNX1 antibody was use for chromatin immunoprecipitation followed by high-throughput sequencing to reveal RUNX1 genome occupancy in hematopoietic stem/progenitor cells. Examination of RUNX1 transcription factor occupancy in EML cells.
Project description:An increasing number of studies, including mutant expression profiling and comparative transcriptomic analyses, require reference RNA-seq data collections in mice. Particularly, to complement previous profiling data sets based on arrays, a full RNA-seq developmental series will be required for whole embryos. E10.5 is a key reference stage as it represents the early organogenesis stage. Here, we have performed high-throughput sequencing of total RNA form whole mice embryos at embryonic stage E10.5.