Transcriptomics,Multiomics

Dataset Information

108

Gene expression profiling of lung cancer cells transfected with scrambled siRNA and siRNA targeting the ETS2 gene


ABSTRACT: ETS2 is a canonical transcriptional factor and member of the ETS family of genes. ETS2 binds to consensus ERE binding sites in a broad spectrum of genes thus affecting many intracellular molecular functions. However, the role of ETS2 in the biology and pathogenesis of lung cancers is still not known. We have found that ETS2 is down-regulated in lung tumors compared to normal lung tissue and the expression of the coding protein of the gene was a significant independent predictor of favorable outcome in NSCLC patients pinpointing to a potential tumor suppressor role for this gene. To better understand its molecular function in NSCLC, we compared and contrasted the transcriptome of lung cancer cells transfected with control siRNA and siRNA targeting ETS2. H441 lung cancer cells were transfected with SMARTpool (Dharmacon)control/scrambled siRNA or siRNA targeting ETS2. Three independent transfections were performed cells with control siRNA and for cells with siRNA specific to ETS2 where each transfection consittutes a biological replicate. Knock-down of ETS2 in all samples was confirmed by quantitative real-time PCR. Total RNA was then profiled using the Human Gene

REANALYSIS of: E-GEOD-43459

ORGANISM(S): Homo sapiens  

PROVIDER: E-GEOD-43459 | ExpressionAtlas | 2015-01-13

REPOSITORIES: ExpressionAtlas

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Publications

ETS2 mediated tumor suppressive function and MET oncogene inhibition in human non-small cell lung cancer.

Kabbout Mohamed M   Garcia Melinda M MM   Fujimoto Junya J   Liu Diane D DD   Woods Denise D   Chow Chi-Wan CW   Mendoza Gabriela G   Momin Amin A AA   James Brian P BP   Solis Luisa L   Behrens Carmen C   Lee J Jack JJ   Wistuba Ignacio I II   Kadara Humam H  

Clinical cancer research : an official journal of the American Association for Cancer Research 20130509 13


The ETS2 transcription factor is an evolutionarily conserved gene that is deregulated in cancer. We analyzed the transcriptome of lung adenocarcinomas and normal lung tissue by expression profiling and found that ETS2 was significantly downregulated in adenocarcinomas. In this study, we probed the yet unknown functional role of ETS2 in lung cancer pathogenesis.Lung adenocarcinomas (n = 80) and normal lung tissues (n = 30) were profiled using the Affymetrix Human Gene 1.0 ST platform. Immunohisto  ...[more]

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