Project description:Transcriptional profiling of human embryo kidney cells comparing control HEK293 transfected with empty vectors cells with HEK293 cells transfected with pcDNA3-ZNF191 (or ZNF191 siRNA vector). We searched for early ZNF191 target genes by using a transient overexpression and knockdown strategy in the human embryo kidney (HEK293) cells. A table of gene targets of transcription factor ZNF191 commonly identified by both strategies is appended below as a supplementary file.
Project description:Werner syndrome (WS) is a rare disorder characterized by the premature onset of a number of age-related diseases. The gene responsible for WS when mutated is believed to be involved in different aspects of transcription, replication, and/or DNA repair. We depleted the human WRN protein in HEK293 cells by transfecting them with small interference RNAs (siRNAs) against the WRN mRNA. The transcription profile of these transfected cells was compared to the transcription profile of HEK293 cells transfected with a scrambled siRNA that does not deplete the WRN protein.
Project description:To investigate the global genes regulated by siIGF2BP3 high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between PC3 cells transfected with control siRNA or siIGF2BP3 siRNA.
Project description:To investigate the global genes regulated by FTO high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between HepG2 cells transfected with control siRNA or FTO siRNA.
Project description:To investigate the global genes regulated by AHR, high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between DLD-1 cells transfected with control siRNA or AHR siRNA.
Project description:To investigate the global genes regulated by lncRNA-SLCC1(RP11-400N13.2), high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between DLD-1 cells transfected with control siRNA or lncSLCC1 siRNA.
Project description:Analysis of differentially expressed genes after siRNA mediated knock-down of the FET-family of proteins; the FUS, EWS, and TAF15 proteins. The FET-proteins are thought to function as transcription factors, and this hypothesis is tested in the present study. Results provide important information of the genes which expression are controlled by the FET-family of proteins. Total RNA obtained HEK293-cells transfected with siRNA targeted against the FUS, EWS, or TAF15 mRNA, or a combination of all three of them. Control cells are transfected with an equal amount of unspecific siRNA without known targets.
Project description:To investigate the global genes regulated by FTO high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between 253Jcells transfected with control siRNA or FTO siRNA.
Project description:To investigate the global genes regulated by METTL3 high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between Huh7cells transfected with control siRNA or METTL3 siRNA.
Project description:HSD3B1 (3beta-hydroxysteroid dehydrogenase type 1) plays a vital role in steroidogenesis. Transcription profiling analysis was performed in MDA-MD-231 breast cancer cells transfected with negative control siRNA or siRNAs against HSD3B1.