Project description:<p>GABAergic interneurons are essential for neural circuit function and their loss or dysfunction is implicated in human neuropsychiatric disease. In vitro methods for interneuron generation hold promise for studying human cellular and functional properties and ultimately therapeutic cell replacement. We used a protocol for generating cortical interneurons from hESCs and analyzed the properties and maturation timecourse of cell types using single-cell RNAseq (data available at <a href="https://www.ncbi.nlm.nih.gov/geo/">GEO</a> under: GSE93802 on March 10 2017). Transcriptomic profiles of the hESC-derived interneurons were compared to several different populations of cells from mid-gestation human neocortex that showed differing levels of <a href="https://www.ncbi.nlm.nih.gov/gene/5080">PAX6</a> and <a href="https://www.ncbi.nlm.nih.gov/gene/6657">SOX2</a> expression.</p> <p>For this study, 104 samples of 100 human neocortical cells each, have been sorted based on <a href="https://www.ncbi.nlm.nih.gov/gene/6657">SOX2</a> and <a href="https://www.ncbi.nlm.nih.gov/gene/5080">PAX6</a> expression, mRNA recovered from the fixed cells by FRISCR, and transcriptomic profiles generated by SmartSeq2. The RNA-seq data from the 104 100-cell samples is included in this dbGaP study.</p>
Project description:To compare miRNA expression profiles between freshly isolated intestinal epithelial cells and cultured organoids in mice. Intestinal organoids largely resembled intestinal epithelial cells in their miRNA profiles. Although the expression levels of some miRNAs were different between crypt and villus epithelial cells, such expression patterns were not reproduced during the maturation of intestinal organoids.