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E-GEOD-51835

ABSTRACT: Effects of exercise on gene expression level in human monocytes

OTHER RELATED OMICS DATASETS IN: PRJNA225579PRJNA225566PRJNA225578

PROVIDER: E-GEOD-51835 | ExpressionAtlas | 2015-07-31

REPOSITORIES: ExpressionAtlas

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Project description:Effects of exercise on gene expression level in human monocytes

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Effects of exercise on microRNA expression level in human monocytes

Project description:Exercise leads to a rapid change in the profile of gene expression in monocytes. We hypothesized that microRNA expression in circulating monocytes would also be affected by brief exercise. Twelve healthy men (22-30yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II, human #130-091-153 and the autoMACSM-BM-. Pro Separator). Total RNA was extracted using TRIzolM-BM-.. For this study we used Agilent Human miRNA microarrays V2 (total of 24 arrays).

2014-02-15 | E-GEOD-51836 | biostudies-arrayexpress

Effects of exercise on gene expression level in human monocytes

Project description:We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in monocytes. Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Monocytes were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human #130-091-153 and the autoMACS√Ç¬Æ Pro Separator). Total RNA was extracted using TRIzol. For this study we used Affymetrix plus 2 (total of 24 chips).

2014-02-15 | E-GEOD-51835 | biostudies-arrayexpress

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