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E-GEOD-53284

ABSTRACT: Transcriptome of HEK293 cells stably knockdown for the BAHD1 gene with a shRNA

OTHER RELATED OMICS DATASETS IN: PRJNA225618PRJNA226087PRJNA231861

PROVIDER: E-GEOD-53284 | ExpressionAtlas | 2016-04-07

REPOSITORIES: ExpressionAtlas

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Transcriptome of HEK293 cells stably knockdown for the BAHD1 gene with a shRNA

Project description:Comparison of gene expression profile of HEK293 cells stably expressing a shRNA control (SilX-CT) or a shRNA against BAHD1 (SilX-BAHD1) We used microarrays to identify the repertoire of genes differentially expressed upon knockdown of the chromatin repressor BAHD1 in human HEK293 cells.

2015-12-11 | E-GEOD-53284 | biostudies-arrayexpress

Transcriptome of HEK293 cells stably knockdown for the BAHD1 gene with a shRNA

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E-GEOD-51863

Project description:Transcriptome of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)

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Project description:Chromatin immunoprecipitation-sequencing of HEK293 cells (HEK293-HPT-blue) and HEK293 cells stably over-expressing the BAHD1 gene (HEK293-HPT-BAHD1)

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Gene expression analysis upon stable SmcHD1 Knock Down

Project description:To study the role of SmcHD1 in regulating gene expression, we have stably knocked down SmcHD1 in HEK293 cells and looked at change in gene expression. shRNA3 and shRNA4 were used to target the expression of the SmcHD1 gene (Genbank Accession number NM_015295). The protein levels of SmcHD1 were knocked down in excess of 90% normal levels in 293 cells. shRNA NC5 a scrambled control shRNA and did not change SmcHD1 protein levels in 293 cells.

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Human OCI-AML3 cells: Control vs. Atg5-shRNA or NPM1-shRNA inducibly expressed

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Project description:Methylated DNA immunoprecipitation-sequencing of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)

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whole-genome bisulfite sequencing (BS-seq) of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)

Project description:Comparison of methylome of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used BS-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.

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