Project description:Exploring miRNA-related antisense transcription in Arabidopsis through RNA transcript profiling of smRNA pathway-defective mutants on a custom high-resolution oligonucleotide array.
2009-04-10 | GSE15199 | GEO
Project description:The transcriptome of TaFLZ2D over-expressing Arabidopsis line
Project description:Transcript profile of apices of 20 days-old Arabidopsis plants over expressing miR396b. We used ATH1 Affymetrics microarrays to obtain the transcription profile of plants overexpressing miR396b.
Project description:RT-PCR analysis of inflammatory cytokines and receptors following Il1alpha treatment of pEGP-miR-135b over-expressing cells compard to controls (pEGP-miR-null) using pathway-specific PCR arrays for the evaluation of mouse inflammatory cytokines and receptors (#PAMM-011D). qPCR array gene expression profiling. This experiment examined the transcriptional response of NIH3T3 cells over-expressing pEGP-miR-135b following IL1alpha treatment. Total RNA (n = 3 per group) was used with SABiosciences pathway-specific PCR arrays for inflammatory cytokines and receptors (#PAMM-011D).
Project description:rs09-07_hb6 - hb6 - The aim is to determine downstream target of HB6 transcription factor involved in plant development - Transcriptome on Arabidopsis HB6 over-expressing plants and BPM Knock down plants during development Keywords: wt vs mutant comparison
2010-08-01 | GSE19095 | GEO
Project description:RNAseq of transgenic Arabidopsis plants over-expressing MSL or FERR
Project description:RNA sequencing of salinity tolerant Arabidopsis thaliana mutants expressing zinc finger artificial transcription factors (ZF-ATFs), with and without salt treatment (0 mM and 75 mM NaCl).
Project description:Global gene expression was compared between Arabidopsis lines with altered expression of ANAC102 (over-expressed and knocked-out) and wild-type. ANAC102 is a putative NAC domain transcription factor. Gene expression was compared between an ANAC102 over-expressing line and parental ecotype C24 under ambient atmosphere to determine which genes ANAC102 is capable of regulating. Gene expression was also compared between three week old plants of an ANAC102 knock-out line and parental ecotype Col-0 under 0.1% Oxygen and ambient atmosphere conditions to determine which genes may require ANAC102 for appropriate expression under these conditions. Gene expression was also compared between imbibed seeds of an ANAC102 knock-out line and parental ecotype Col-0 following a 0.1% Oxygen treatment. Experiment Overall Design: Three related experiments were performed. In the first two, three week old Arabidopsis seedlings (4-6 leaves) were used. In the first experiment one line of ANAC102 over-expressing plants were compared to ecotype C24 (the parental line used in the creation of the ANAC102 over-expressing line). Two biological replicates were used for each line and for each biological replicate, five Arabidopsis seedlings grown on the same petri dish were bulked. RNA was extracted from whole plants. In the second experiment, one line of plants bearing a T-DNA insertion in the second exon of the ANAC102 gene was compared to ecotype Col-0 (the parental line used for the T-DNA mutagenesis) both without treatment and following treatment with 0.1% Oxygen for four hours. Two biological replicates were used for each line and for each biological replicate, five Arabidopsis seedlings grown on the same petri dish were bulked. RNA was extracted from root tissue only. In the third experiment imbibed seeds of one Arabiopsis line bearing a T-DNA insertion in the second exon of the ANAC102 gene was compared to seeds of ecotype Col-0 (the parental line used for the T-DNA mutagenesis) following treatment with 0.1% Oxygen for six days. Three biological replicates were used for each line and for each biological replicate, ~5mg (pre-imbibition) of seed were bulked. RNA was extracted from whole seed.