E-GEOD-5539
Ontology highlight
ABSTRACT:
PROVIDER: E-GEOD-5539 | ExpressionAtlas | 2014-07-03
REPOSITORIES: ExpressionAtlas
Similar Datasets
Project description:Over-expression of MBF1c enhances stress tolerance
| PRJNA96027 | ENA
Project description:Multiprotein bridging factor 1c MBF1c (At3g24500) is a stress-response transcription co-activator. To test the function of MBF1c, we over-expressed it in transgenic Arabidopsis plants using the 35S-CaMV promoter. T4 seeds form 3 independent lines were tested for their tolerance to biotic and abiotic stress conditions. Constitutive expression of MBF1c in Arabidopsis enhanced the tolerance of transgenic plants to bacterial infection, salinity, heat and osmotic stress. Moreover, the enhanced tolerance of transgenic plants to osmotic and heat stress was maintained even when these two stresses were combined. The expression of MBF1c in transgenic plants augmented the accumulation of a number of sugars and defense transcrtipts in response to heat stress. Transcriptome profiling and inhibitor studies suggest that MBF1c expression enhances the tolerance of transgenic plants to heat and osmotic stress by partially activating, or perturbing, the ethylene-response signal transduction pathway. MBF1 proteins could be used to enhance the tolerance of plants to different abiotic stresses. Suzuki et al., 2005 Plant Physiology, submitted. Experimenter name = Ron Mittler; Experimenter phone = 1-775-784-1384; Experimenter fax = 1-775-784-1650; Experimenter department = Dept. of Biochemistry; Experimenter institute = University of Nevada; Experimenter address = MS200; Experimenter address = Reno; Experimenter address = Nevada; Experimenter zip/postal_code = 89557; Experimenter country = USA Experiment Overall Design: 6 samples were used in this experiment
2008-06-13 | E-GEOD-5539 | biostudies-arrayexpress
Project description:Melatonin enhances plant growth and abiotic stress tolerance in soybean plants
| PRJNA248556 | ENA
Project description:Multiprotein bridging factor 1c MBF1c (At3g24500) is a stress-response transcription co-activator. To test the function of MBF1c, we over-expressed it in transgenic Arabidopsis plants using the 35S-CaMV promoter. T4 seeds form 3 independent lines were tested for their tolerance to biotic and abiotic stress conditions. Constitutive expression of MBF1c in Arabidopsis enhanced the tolerance of transgenic plants to bacterial infection, salinity, heat and osmotic stress. Moreover, the enhanced tolerance of transgenic plants to osmotic and heat stress was maintained even when these two stresses were combined. The expression of MBF1c in transgenic plants augmented the accumulation of a number of sugars and defense transcrtipts in response to heat stress. Transcriptome profiling and inhibitor studies suggest that MBF1c expression enhances the tolerance of transgenic plants to heat and osmotic stress by partially activating, or perturbing, the ethylene-response signal transduction pathway. MBF1 proteins could be used to enhance the tolerance of plants to different abiotic stresses. Suzuki et al., 2005 Plant Physiology, submitted. Experimenter name = Ron Mittler Experimenter phone = 1-775-784-1384 Experimenter fax = 1-775-784-1650 Experimenter department = Dept. of Biochemistry Experimenter institute = University of Nevada Experimenter address = MS200 Experimenter address = Reno Experimenter address = Nevada Experimenter zip/postal_code = 89557 Experimenter country = USA Keywords: genetic_modification_design; stimulus_or_stress_design
2006-08-15 | GSE5539 | GEO
Project description:Plant homeodomain (PHD) finger proteins are ‘histone code readers’. They recognize and bind to epigenetically modified histone H3 ‘tail’. Here we reported that an Alfin1-like soybean protein, GmPHD6, read H3K4me0/1/2 but not H3K4me3 with the N-terminal instead of the PHD finger. GmPHD6 does not possess transcriptional regulatory ability. Through the PHD finger, GmPHD6 interacts with its co-activator, LHP1-1/2. Using a transgenic hairy root system, we demonstrated that over-expression of GmPHD6 improved stress tolerance in transgenic soybean composites. Perhaps due to the excessive amount of LHP1 compared to that of GmPHD6, over-expression of LHP1-1/2 failed to do so. The integrity of the complex is essential in stress response, for the abrogation of DNA binding activity of GmPHD6 or the decrease of LHP1 content leads to stress sensitivity in soybean. GmPHD6 influences expression of dozens of stress-related genes to confer stress tolerance. Among these genes, we identified three direct targets of GmPHD6, ASR (ABA-stress-ripening induced), CYP71A22 (cytochrome P450) and CYP82C4. Our study reveals significant findings in stress response. GmPHD6 and LHP1 are recruited to H3K4me0/1/2 marks, where several stress-related genes may locate in, to form a transcriptional activation complex. GmPHD6 locates target sites through recognizing the G-rich elements in their promoters; LHP1 enhances expression levels of these targets. Genetically engineering of GmPHD6/LHP1 complex should improve stress tolerance in crop plants.
2018-02-13 | GSE85077 | GEO
Project description:To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress DREB1A and GA5. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and GA5 encodes a GA synthetic enzyme which enhances plant growth. We performed microarray experiments using a single overexpressor of GA5 and the double overexpressor of DREB1A and GA5 to study the potential interactions of the gene networks regulated by the two factors in the double overexpressor.
2020-02-29 | E-MTAB-6550 | biostudies-arrayexpress
Project description:To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress two transcription factors: DREB1A and PIF4. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and PIF4 enhances cell elongation through activation of cell wall synthesis. We performed microarray experiments using a single overexpressor of PIF4 and the double overexpressor of DREB1A and PIF4 to study the potential interactions of the gene networks regulated by the two transcription factors in the double overexpressor.
2019-01-07 | E-MTAB-6549 | biostudies-arrayexpress
Project description:To improve both drought stress tolerance and growth of plants, we generated transgenic Arabidopsis plants that overexpress two transcription factors: DREB1A and OsPIL1. It was reported that DREB1A improves drought stress tolerance in various crops but causes dwarfism, and OsPIL1 enhances cell elongation through activation of cell wall synthesis in rice. We performed microarray experiments using a single overexpressor of OsPIL1 and the double overexpressor of DREB1A and OsPIL1 to study the potential interactions of the gene networks regulated by the two transcription factors in the double overexpressor.
2016-12-06 | E-MTAB-4073 | biostudies-arrayexpress
Project description:The purine metabolite allantoin enhances abiotic stress tolerance through synergistic activation of abscisic acid metabolism
| PRJNA192529 | ENA