Project description:Mitochondrial stress stimuli such as AA caused a transient suppression of auxin signaling and conversely, auxin treatment represses a part of the response to AA treatment. Expression data of Col:LUC Arabidopsis treated with antimycin A (AA) in the presence or absence of a synthetic auxin analogue
Project description:Mitochondrial stress stimuli such as AA caused a transient suppression of auxin signaling and conversely, auxin treatment represses a part of the response to AA treatment. Expression data of Col:LUC Arabidopsis treated with antimycin A (AA) in the presence or absence of a synthetic auxin analogue Total RNA extraction was carried out on 80 mg of Col:LUC Arabidopsis seedlings grown on B5 media for 10 days and transferred to fresh plates, containing either B5 media or B5 media supplemented with 4.5 µM NAA for an additional 3 days. Seedlings were treated with 50 µM AA for 3 hours.
Project description:The aim of the project is to compare NAA-induced proteome remodelling between wild-type plants (Arabidopsis thaliana, Col-0) to autophagy deficient mutants (atg2-1) treated MS growt media suplemented with solvent (EtOH- Control) or NAA (a synthetic variant of the plant hormone auxin. The aim is to look at the rapid response when treated with auxin and as so the treatment is very short at 30 minutes. The time of the experiment was at zeitgeber 0.
Project description:Analysis of brassinosteroid (BR) and auxin effects on gene expression in Arabidopsis roots. Our genomic results indicate that BR and auxin induce largely opposite gene expression responses in primary roots. RNA-Seq for 7-day-old Arabidopsis Col-0, dwf4, bri1-116, and bri1-116;bzr1-1D roots grown on regular medium and treated with brassinolide, auxin or mock solution for 4 hr.
Project description:After analysing auxin metabolism in auxin dependent tobacco BY-2 cell line grown in presence or absence of synthetic auxin 2,4-D we found that both conditions were similarly characterized by very low levels of endogenous indole-3-acetic acid (IAA) and its metabolites. However, metabolic profiling after exogenous application of IAA uncovered that the concentration of N-(2-oxindole-3-acetyl)-L-aspartic acid (oxIAA-Asp), the most abundantly formed auxin metabolite in the control culture, dramatically decreased in auxin-starved conditions. To describe the molecular mechanism behind this regulation, we analysed transcriptome and proteome changes caused by auxin starvation. While no changes in the expression of auxin biosynthetic machinery were observed, many genes related to auxin conjugation and degradation showed differential expression. Selected putative auxin glycosylating enzymes as well as members of the Gretchen Hagen 3 gene family involved in auxin amino acid conjugation showed both up- and down-regulation. Contrarily to that, all tobacco homologs of Arabidopsis thaliana DIOXYGENASE FOR AUXIN OXIDATION 1 (DAO1), known to be responsible for the formation of oxIAA from IAA, showed significant downregulation at both transcript and protein levels.
Project description:In this study we profile expression of target genes downstream of auxin signaling in Arabidopsis hypocotyls elongating in response to auxin. The synthetic auxin picloram is used in order to take advantage of the picloram-resistant auxin receptor mutant afb5-5, which fails to elongate in response to picloram.
Project description:Above ground tissue of 10 day old Arabidopsis seedlings of Col wild-type, 35S-ARR7, arr7, 35S-ARR15 was treated with Cytokinin (benzyladenine), Auxin (indole-3-acetic acid) or both.
Project description:To obtain more information on auxin-regulated gene expression, we treated Arabidopsis seedlings with auxin biosynthesis or signaling inhibitors, then performed DNA microarray analyses.
Project description:Roots of 5-day-old 35S::WRKY23-GR were treated for 6 hours with 10 μM DEX or DMSO, respectively. Wt Col-0 and 35S::WRKY23-SRDX plants were treated with 10 μM NAA or DMSO for 6 hours. Roots were subsequently collected for RNA isolation. All replicates were sampled in three independent experiments. Total RNA (200 μg per array) was hybridized to ATH1 Affymetrix Arabidopsis arrays in accordance with the standard procedures at the VIB Nucleomics Core. Data files containing probe level intensities (.cel files) were used for background correction and normalization was done in R (http://www.r-project.org) using the Bioconductor package affylmGUI (http://bioinf.wehi.edu.au/affylmGUI/) using the log2 scale RMA procedure. Genes with the same or contrasting WRKY23 expression profiles were selected by Pavlidis template matching in TMeV 4.0 (TIGR) (28). Finally, genes with a significant p value (< 0.001), denoting expression above background with minimum 2-fold change compared to the respective control, were retained for further analysis. First, by comparing 35S::WRKY23-GR roots with and without induction by dexamethasone, we obtained a set of 110 genes as potential WRKY23 targets, which were up-regulated in dexamethasone-treated seedlings. Next, we identified 950 genes, which were auxin-inducible in Col-0 wild type (Wt), but lost this auxin responsiveness in dominant-negative 35S::WRKY23-SRDX roots. The overlap between the two datasets yielded a list of 61 genes (Fig. S1B). Because WRKY23 acts downstream of the SLR/IAA14 transcriptional repressor, this genelist was compared with previously published microarray data on auxin-treated solitary root1 (slr-1) mutant seedlings