Project description:To understand the global effect of Gln on gene expression, we performed RNA-seq analysis to compare the gene expression profiles between 12-day-old Arabidopsis seedlings grown on 5 mM NH4NO3 and 5 mM Gln as the sole N source.
Project description:Ethylene-dependent gene expression was assayed by treating with 1 uM ACC, an ethylene precursor, or a control treatment to Arabidopsis seedlings by transferring 6 day old Arabidopsis grown on a a nylon mesh to fresh ACC-containing or control media Seedling roots were harvested 0, .5, 1, 2, 4, 8, 12, and 24 hours after treatment and the resultant RNA was used for microarray analysis to determine the kinetic profiles of auxin-responsive gene expression