Transcriptomics,Multiomics

Dataset Information

122

Expression data from 24 hours of Sox17 overexpression in pancreatic islets of a 16-week old mice

ABSTRACT: Secretion of insulin by pancreatic β cells in response to glucose is central for glucose homeostasis, and dysregulation of this process is a hallmark of the early stages of diabetes. We utilized a tetracycline-inducible approach to investigate the immediate impact of a pulse of Sox17 expression on the insulin secretory pathway. Sox17 gain-of-function animals (Sox17-GOF) were generated using an Ins2-rtTA mouse line and a line in which Sox17 expression is regulated by the tetracycline transactivator (tetO-Sox17). Administering doxycycline to 16-week old mice resulted in Sox17 overexpression in mature β cells in the islets. In order to identify the molecular basis by which Sox17 regulates the secretory pathway in β cells, we performed microarray analysis on isolated islets following a 24-hour pulse of Sox17 overexpression. We chose to analyze a 24 hour pulse of Sox17 for islet pancreas RNA extraction and hybridization on Affymetrix microarrays since it was sufficient to stimulate the insulin secretory pathway without causing changes in islet architecture.

REANALYSIS of: E-GEOD-59928

ORGANISM(S): Mus musculus  

PROVIDER: E-GEOD-59928 | ExpressionAtlas | 2015-07-29

REPOSITORIES: ExpressionAtlas

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Publications

Sox17 regulates insulin secretion in the normal and pathologic mouse β cell.

Jonatan Diva D   Spence Jason R JR   Method Anna M AM   Kofron Matthew M   Sinagoga Katie K   Haataja Leena L   Arvan Peter P   Deutsch Gail H GH   Wells James M JM  

PloS one 20140821 8

SOX17 is a key transcriptional regulator that can act by regulating other transcription factors including HNF1β and FOXA2, which are known to regulate postnatal β cell function. Given this, we investigated the role of SOX17 in the developing and postnatal pancreas and found a novel role for SOX17 in regulating insulin secretion. Deletion of the Sox17 gene in the pancreas (Sox17-paLOF) had no observable impact on pancreas development. However, Sox17-paLOF mice had higher islet proinsulin protein  ...[more]

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