Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-60183

ABSTRACT: Gene expression in the epidermis including guard cells of phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2

OTHER RELATED OMICS DATASETS IN: PRJNA257692

PROVIDER: E-GEOD-60183 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-60183-analysis-methods.tsv	Tabular
	E-GEOD-60183-analytics.tsv	Tabular
	E-GEOD-60183-analytics.tsv.undecorated	Tabular
	E-GEOD-60183-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-60183-analytics.tsv.unrounded	Tabular

Items per page:

1 - 5 of 23

Similar Datasets

Gene expression in the epidermis including guard cells of phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2

2015-02-04 | GSE60183 | GEO

Gene expression in the epidermis including guard cells of phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2

Project description:Stomata pores are surrounded by a pair of guard cells in the epidermis in plants. Stomata apature is controlled by environmental factors. Stomata opening is also enhanced by introduction of SOC1-GFP driven by GC1 promoter in phot1 phot2 double mutants (pGC1::SOC1-GFP/ phot1 phot2). Epidermis including guard cells were isolated from phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2 and gene expression were analyzed. phot1-5 phot2-1 in gl1 background (phot1 phot2) and pGC1::SOC1-GFP/phot1 phot2 were grown under 16 h light / 8h dark, constant 22?C conditions for 4 to 5 weeks. Epidermis including guard cells were isolated from leaves of these plants. Three biological replicates were used.

2015-02-04 | E-GEOD-60183 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Gene expression in the epidermis including guard cells of phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2

| PRJNA257692 | ENA

E-GEOD-29137

Project description:Expression profile of MDA-MB-231 and MCF7 cells stably transfected with GFP-Numb4, GFP-Numb5, GFP-Numb6

2025-01-24 | E-GEOD-29137 | ExpressionAtlas

E-GEOD-50226

Project description:Gene expression data from 1833 cells and 1833 cells depleted of BACH1 mRNA expression

2025-01-24 | E-GEOD-50226 | ExpressionAtlas

E-GEOD-58351

Project description:Gene expression in intestinal stem cells

2014-07-30 | E-GEOD-58351 | ExpressionAtlas

E-GEOD-38007

Project description:Gene expression profile of MITF expressing cells

2025-01-24 | E-GEOD-38007 | ExpressionAtlas

E-GEOD-53894

Project description:G9a-dependent gene expression in mouse AML cells

2015-07-31 | E-GEOD-53894 | ExpressionAtlas

Co-immunoprecipitation of FAMA-interacting proteins in Arabidopsis thaliana seedlings

Project description:Identifying interaction partners and protein complex compositions for transcription factors (TFs) can produce valuable information on the mechanisms by which they regulate gene expression or are themselves regulated by signaling pathways in the cell. FAMA is a TF that is specifically expressed in the last stages of stomatal guard cell development in the epidermis of young leaves and other aerial tissues of higher plants. It is a master regulator of guard cell development and promotes terminal differentiation of the guard cell precursor by both activating and repressing hundreds of genes. How this is achieved mechanistically is still unclear. We therefore isolated putative FAMA interaction partners from young Arabidopsis seedlings expressing FAMA-CFP under the FAMA promoter using proteasome inhibitor treatment and formaldehyde crosslinking, followed by a classical co-immunoprecipitation mass spectrometry approach. Plants expressing nuclear GFP under a stomatal lineage specific promoter were used as controls. In two independent experiments with different crosslinking and immunoprecipitation conditions, we found ICE1, a known heterodimerization partner of FAMA, to be enriched in FAMA-CFP versus control samples. Other proteins with known transcriptional regulator or co-regulator function were not identified, presumably due to the low abundance of the bait protein. The interaction of FAMA with BRXL2, another regulator of guard cell development, which was detected in one experiment, is likely an artifact since BRXL2 does not localize to the nucleus under normal conditions.

2019-09-26 | PXD015212 | Pride

Analysis of phot2 phosphorylation profiles and interaction partners involved in chloroplast accumulation and avoidance responses in Arabidopsis thaliana

Project description:The project aims to use photocycle mutants to understand the importance of phot2 autophosphorylation for signaling leading to chloroplast accumulation and avoidance. The second part is focused on the identification of new interacting proteins for phot2. Characterization of proteins important for signaling leading to chloroplast avoidance. PHOT2-GFP wild type and PHOT2-V392L-GFP muteins were immunoprecipitated from leaves of transgenic Arabidopsis plants, either dark-adapted or irradiated with low or high blue light. Proteins identified by Mass Spectrometry as interacting with wild-type PHOT2-GFP and PHOT2-V392L-GFP were compared to identify proteins putatively involved in chloroplast avoidance. Phosphorylation profiles of PHOT2-GFP wild type and PHOT2-V392L-GFP were analyzed to distinguish between phosphorylation sites characteristic for chloroplast accumulation and avoidance.

2024-03-13 | MSV000094308 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data