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E-GEOD-61243

ABSTRACT: Expression data from yeast heterologously expressing alpha-ENaC, CFTR or Kir2.1 alpha-ENaC]

OTHER RELATED OMICS DATASETS IN: PRJNA260581PRJNA260578PRJNA260580PRJNA260582

PROVIDER: E-GEOD-61243 | ExpressionAtlas | 2015-08-05

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-61243-analysis-methods.tsv	Tabular
	E-GEOD-61243-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-61243-configuration.xml	Xml
	E-GEOD-61243-percentile-ranks.tsv	Tabular
	E-GEOD-61243.condensed-sdrf.tsv	Tabular

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E-GEOD-61244

Project description:Expression data from yeast heterologously expressing alpha-ENaC, CFTR or Kir2.1 [CFTR]

2015-08-06 | E-GEOD-61244 | ExpressionAtlas

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Project description:Expression data from yeast heterologously expressing αENaC, CFTR or Kir2.1 [Kir2.1]

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Extrarenal α-klotho expression in human tissues

Project description:α-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane α-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. Results: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane α-Klotho isoform. Conclusions: The data presented confirms α-Klotho expression in the kidney tubule and in artery, and provides evidence of α-Klotho expression across organ systems and cell-types that have not previously been described in humans.

2015-10-13 | PXD002775 | Pride

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