Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-63015

ABSTRACT: Transcription profiling by high throughput sequencing of human polarized cerebellar plate neuroepithelium in three-dimensional culture

OTHER RELATED OMICS DATASETS IN: PRJNA266431

PROVIDER: E-GEOD-63015 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-63015-analysis-methods.tsv	Tabular
	E-GEOD-63015-analytics.tsv	Tabular
	E-GEOD-63015-analytics.tsv.undecorated	Tabular
	E-GEOD-63015-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-63015-analytics.tsv.unrounded	Tabular

Items per page:

1 - 5 of 33

Similar Datasets

Self-organization of polarized cerebellar plate neuroepithelium in three-dimensional culture of human pluripotent stem cells

Project description:During cerebellar development, the main portion of the cerebellar plate neuroepithelium (NE) gives birth to Purkinje cells and interneurons, while the germinal zone at its dorsal edge, called the rhombic lip (RL), generates granule cells and cerebellar nuclei neurons. However, it remains elusive how these components work together to generate the intricate structure of the cerebellar anlage. In this study, we found that a polarized cerebellar anlage structure self-organizes in three-dimensional (3D) human ES cell (hESC) culture. This NE is capable of differentiating into electrophysiologically functional Purkinje cells. The addition of FGF19 promotes spontaneous generation of dorsoventrally polarized NE structures containing cerebellar and basal plates. Furthermore, further addition of SDF1 promoted the generation of stratified cerebellar plate NE with RL-like germinal zones self-forming at the edge. Thus, hESC-derived cerebellar progenitors exhibit substantial self-organizing potential for generating a polarized structure reminiscent of the early human cerebellar anlage at the first trimester. Examination of mRNA profile in two different treated human ES cells .

2015-04-15 | E-GEOD-63015 | biostudies-arrayexpress

Self-organization of polarized cerebellar plate neuroepithelium in three-dimensional culture of human pluripotent stem cells

2015-04-15 | GSE63015 | GEO

Homo sapiens

Project description:Self-organization of polarized cerebellar plate neuroepithelium in three-dimensional culture of human pluripotent stem cells

| PRJNA266431 | ENA

Analysis of 3D human airway epithelial cultures by single-cell RNA-seq

Project description:We report the application of single-cell-based sequencing technology for high-throughput profiling of cell types and and transcriptional state of cells in the complex tissue of the human airway epithelium. Our model system is that of polarized human airway epithelial cultures, differentiated from hTert-immortalized basal-like precursor cells.

2021-02-11 | GSE166601 | GEO

Transcription profiling by high throughput sequencing of MPN1 deficient human cells

Project description:Transcription profiling by high throughput sequencing of MPN1 deficient human cells

| PRJEB3131 | ENA

miRNA sequencing from exosomes derived from M1-or M2-polarized bone-marrow derived macrophages (BMDMs).

Project description:We reported exosome-guided phenotype switches between M1- and M2-polarized BMDMs. M1- or M2-polarized BMDMs were successfully reprogrammed to M2- or M1-phenotype via the treatment of exosomes obtained from M2- or M1-polarized BMDMs. In this uploaded information, the exosomes from M1- and M2-polarized BMDMs were analyzed by high-throughput sequencing.

2022-03-02 | GSE179172 | GEO

To explore the relationship between new gene expression / mutation and survival of patients in colorectal cancer

Project description:Interventions: Case vs control:No Primary outcome(s): High-throughput sequencing Study Design: Case-Control study

| 2676067 | ecrin-mdr-crc

RNA-Seq of human individual tissues and mixture of 16 tissues (Illumina Body Map)

Project description:Transcription profiling by high throughput sequencing of individual and mixture of 16 human tissues RNA. This dataset is part of the TransQST collection.

2011-05-05 | E-MTAB-513 | biostudies-arrayexpress

Identification of genes responsive to change in apical polarity in the breast epithelium

Project description:Differentiation of monolayered epithelia is characterized by the formation of a basoapical polarity axis, except during the early stages of cancer development. Using mammary glandular structures (acini) produced in a three-dimensional cell culture system we have demonstrated that, in order for mammary epithelial cells to exit quiescence and enter the cell cycle, acini have to lose apical polarity. In order to identify the genes dependent on apical polarity that could control cell quiescence, and possibly other aspects of tissue homeostasis, we have used Affymetrix technology microarray analysis of the 22,277 features/genes of the Human Genome U133A 2.0 array Chip in apically polarized and non-polarized breast epithelial acinar cells in three-dimensional culture. Genes commonly down-regulated in two treatments that altered apical polarity compared to control were considered to be dependent on apical polarity status for their transcription. Apically polarized (Control) and two different populations of non-polarized human mammary epithelial cells (18-alpha-glycyrrhetinic acid =AGA, and 5-aza-2'-deoxycytidine =Aza) were used for microarray analysis containing four biological replicates (1-4) for each treatment. The two treatments that cause loss of apical polarity were compared to reference apically polarized control samples using two different statistical analysis methods (FDR and HolmM-bM-^@M-^Ys).

2012-02-29 | E-GEOD-35165 | biostudies-arrayexpress

Identification of genes responsive to change in apical polarity in the breast epithelium

2012-02-29 | GSE35165 | GEO