Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-68684

ABSTRACT: Transcription profiling by high throughput sequencing of Arabidopsis transgenic plants expressing 35S::FLAG-GR-KAN1 exposed to either mock treatment or dexamethasone (DEX) to induce KAN1 release from its cytoplasmic blockage

PROVIDER: E-GEOD-68684 | ExpressionAtlas | 2020-04-15

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-68684-analysis-methods.tsv	Tabular
	E-GEOD-68684-analytics.tsv	Tabular
	E-GEOD-68684-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-68684-configuration.xml	Xml
	E-GEOD-68684-g1_g2-mvaPlot.png	Other

Items per page:

1 - 5 of 20

Similar Datasets

Arabidopsis Forever Young Flower (FYF): 35S::FYF+GR

Project description:Transcriptional profiling of 35S::FYF+GR transgenic plant's floral buds(DEX treat) compared to 35S::FYF+GR transgenic plant's floral buds(mock treat). DEX : Dexamethasone

2015-06-01 | GSE69411 | GEO

High-thoughput Illumina RNA sequencing to identify downstream target genes of RABBIT EARS (RBE) in the flowers of Arabidopsis thaliana

Project description:In order to identify putative downstream target genes of RBE, we sequenced mRNA from dexamethasone (DEX) and mock treated transgenic Arabidopsis line 35S:GR-RBE (RBE coding region fused to a glucocorticoid receptor domain driven by the constitutive 35S promoter) floral tissues. We compared the results from DEX and mock treatments and focused on the 832 genes whose expression was significantly reduced (P < 0.025) by 2-fold or more in DEX as compared to mock-treated plants. In this analysis, we identified MIR164c (EEP1) as a candidate target of RBE, which was further confirmed by other molecular and genetic analyses. Regulation of MIR164c by RBE is important for normal floral organ formation in Arabidopsis.

2012-07-12 | GSE36469 | GEO

Ath_Agilent_v2_Riechmann array expression profile AP1-GR ap1cal inflorescences - chx experiment

Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone or dexamethasone+cycloheximide treatment. Tissue samples were collected at 3hrs after the treatment. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (Agilent, custom-commercial). We treated inflorescences of 35S:AP1-GR ap1-1 cal-1 plants with a dexamethasone-containing or a mock solution, or with identical solutions that contained in addition 10 M-NM-<M cycloheximide. Tissue was collected 3 hours after the treatment. Samples from each of the four biological replicates resulted in a set of four hybridization pairs: Mock vs. Dex, Mock vs. Chx, Mock vs. Dex+Chx, and Chx vs. Dex+Chx. Dye polarities were switched between biological replicates.

2011-01-01 | E-GEOD-20175 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis thaliana shoots treated with either DEX or CHX to identify STM-regulated target genes

Project description:Global expression of the Arabidopsis transcriptional regulator SHOOT MERISTEMLESS (STM) in a 35S:STM-GR transgenic line was induced for 3 hours with dexamethasone (DEX) compared to mock-induced (DMSO) control. Additionally,to identify direct targets, plants were treated with the protein synthesis inhibitor cycloheximide (CHX) together with DEX, compared to CHX treatment alone.

2018-05-29 | E-MTAB-6786 | biostudies-arrayexpress

Transcription profiling of A. thaliana L.er vs fe-1

Project description:The Arabidopsis thaliana Myb transcription factor, FE, acts as a key regulator of phase transition. In order to identify potential target genes of FE protein, we performed microarray experiments. Using fe-1 and transgenic plants overexpressing GR-tagged FE (35S::FE-GR), we compared transcriptional profiling of WT (L.er) vs fe-1 and Dex-treated 35S::FE-GR vs Mock-treated 35S::FE-GR. Transcriptional profiling of A. thaliana comparing WT (L.er) with the fe-1 mutant

2017-01-01 | GSE42862 | GEO

Isolation of poplar genes involved in xylem vessel differentiation

Project description:Downstream genes of PtVNS genes were explored with inducible expression system using glucocorticoid receptor (GR). Transgenic poplar plants expressing 35S:AtVND7-VP16-GR were treated with dexamethazone (DEX). A number of genes related to the formation of xylem vessels were induced by DEX.

2011-05-01 | GSE25304 | GEO

Isolation of poplar genes involved in xylem vessel differentiation

2011-04-30 | E-GEOD-25304 | biostudies-arrayexpress

Finding direct target genes of VND7

Project description:Direct target genes of VND7 were explored with inducible expression system using glucocorticoid receptor (GR). Transgenic plants expressing 35S:VND7-VP16-GR were treated with dexamethazone (DEX) and/or protein synthesis inhibitor cycloheximide (CHX). A number of genes related to the formation of vascular vessel was induced by DEX even in the presence of CHX. Total RNAs of the transgenic plants expressing 35S:VND7-VP16-GR treated with DEX plus CHX and those treated with CHX only were compared. As a control experiment, transgenic plants harboring empty vector were treated similarly and the total RNAs were compared similarly to identify genes merely induced by DEX treatment itself.

2011-03-31 | E-GEOD-24169 | biostudies-arrayexpress

Identification of putative targets of AP3/PI

Project description:To identify genes regulated by AP3/PI, we carried out microarray experiments using an Arabidopsis whole genome GeneChip array (ATH1 GeneChip, Affymetrix, Santa Clara, CA) in conjunction with an inducible AP3-GR system. For these experiments, we used 35S::AP3-GR transgenic plants in a 35S::PI, ap3-3 null mutant background for various dex or mock treatments. RNA was extracted from inflorescences at 0 and 4 hours after dex or a mock treatment and used as probes for our microarray experiments. Three biological replicates of each were hybridized to Affymetrix ATH1 arrays. We used the Affymetrix Microarray Suite software (MAS) to identify genes whose expression profiles changed only after dex-treatment and are likely targets of AP3/PI. Keywords: time course

2007-11-28 | GSE9702 | GEO

Analysis of genes regulated by an Arabidopsis transcription factor, Dof3.2

Project description:To obtain information on which genes are regulated by an Arabidopsis transcription factor Dof3.2, we treated Arabidopsis transgenic 35S::Dof3.2-GR seedlings with dexamethasone (DEX), then performed DNA microarray analyses. T3 homozygous Dof3.2-GR transgenic line was used.

2020-03-01 | GSE58064 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data