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E-GEOD-71312

ABSTRACT: Expression data from WT Col-0 and the pdx1.3 ko mutant of Arabidopsis

OTHER RELATED OMICS DATASETS IN: PRJNA290882

PROVIDER: E-GEOD-71312 | ExpressionAtlas | 2015-09-18

REPOSITORIES: ExpressionAtlas

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Dataset's files

Source:

			Action	DRS
	E-GEOD-71312-analysis-methods.tsv	Tabular
	E-GEOD-71312-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-71312-configuration.xml	Xml
	E-GEOD-71312-percentile-ranks.tsv	Tabular
	E-GEOD-71312.condensed-sdrf.tsv	Tabular

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Project description:Expression data from WT Col-0 and the pdx1.3 ko mutant of Arabidopsis

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Expression data from WT Col-0 and the pdx1.3 ko mutant of Arabidopsis

Project description:We performed a microarray experiment to assess the global changes in transcription occurring in leaves and roots of the vitamin B6 deficient pdx1.3 knockout mutant in comparison to WT. Vitamin B6 (pyridoxal 5′-phosphate) is an essential cofactor of many metabolic enzymes. Plants biosynthesize the vitamin de novo employing two enzymes, pyridoxine synthase1 (PDX1) and PDX2. In Arabidopsis (Arabidopsis thaliana), there are two catalytically active paralogs of PDX1 (PDX1.1 and PDX1.3) producing the vitamin at comparable rates. Since single mutants are viable but the pdx1.1 pdx1.3 double mutant is lethal, the corresponding enzymes seem redundant. However, the single mutants exhibit substantial phenotypic differences, particularly at the level of root development, with pdx1.3 being more impaired than pdx1.1. Here, we investigate the impact of possible global changes in gene expression in the pdx1.3 mutant compared to WT on the phenotype.

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Project description:The gene expression profiles of WT and NML-KO livers

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Arabidopsis leaves GC-MS profiling for the study of OXI1 (AT3G25250.1)

Project description:GC-MS profiles of Arabidopsis full rosettes methanolic extracts, derivatized by methoxyamine and BSTFA. WT= Col-0; KO mutant oxi1-2 and two OXI1 overexpressing lines. OXI1 is a kinase involved in plant immunity that controls the phytohormon pipecolic acid.

2022-10-02 | MSV000090447 | MassIVE

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