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E-MEXP-1146

ABSTRACT: Transcription profiling of mouse in vivo matured MII oocytes and fully in vivo grown germinal vesicle oocytes to identify gene transcripts linked to epigenetic reprogramming

PROVIDER: E-MEXP-1146 | ExpressionAtlas | 2015-10-22

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MEXP-1146-analysis-methods.tsv	Tabular
	E-MEXP-1146-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-1146-configuration.xml	Xml
	E-MEXP-1146-percentile-ranks.tsv	Tabular
	E-MEXP-1146.condensed-sdrf.tsv	Tabular

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Transcription profiling of mouse in vivo matured MII oocytes and fully in vivo grown germinal vesicle oocytes to identify gene transcripts linked to epigenetic reprogramming

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Transcription profiling of immature germinal vesicle stage oocytes with mature metaphase II oocytes to identify and characterize the changed and stable transcripts during mouse oocyte maturation.

Project description:There is massive destruction of transcripts during maturation of mouse oocytes. The objective of this project was to identify and characterize the transcripts that are degraded versus those that are stable during the transcriptionally silent germinal vesicle (GV)-stage to metaphase II (MII)-stage transition using the microarray approach. A system for oocyte transcript amplification using both internal and 3'-poly(A) priming was utilized to minimize the impact of complex variations in transcript polyadenylation prevalent during this transition. Transcripts were identified and quantified using Affymetrix Mouse Genome 430 v2.0 GeneChip. The significantly changed and stable transcripts were analyzed using Ingenuity Pathways Analysis and GenMAPP/MAPPFinder to characterize the biological themes underlying global changes in oocyte transcripts during maturation. It was concluded that the destruction of transcripts during the GV to MII transition is a selective rather than promiscuous process in mouse oocytes. In general, transcripts involved in processes that are associated with meiotic arrest at the GV-stage and the progression of oocyte maturation, such as oxidative phosphorylation, energy production, and protein synthesis and metabolism, were dramatically degraded. In contrast, transcripts encoding participants in signaling pathways essential for maintaining the unique characteristics of the MII-arrested oocyte, such as those involved in protein kinase pathways, were the most prominent among those stables. Experiment Overall Design: Comparison immature GV-stage oocyte (3 biological replicates) with mature MII-stage oocytes (3 biological replicates)

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Porcine oocytes matured in a chemically defined medium are transcriptionally active

Project description:The statement that fully-grown porcine oocytes are transcriptionally quiescent is not as strong as before. Currently, we know that there is a difference in the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and metaphase (MII) oocytes cultivated in a chemically defined, FLI medium. Oocytes were sequenced and subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR).

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