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E-MEXP-1524

ABSTRACT: Transcription profiling of grapevine cell culture treated with methyly jasmonate, salicylic acids and ethanol controls

PROVIDER: E-MEXP-1524 | ExpressionAtlas | 2015-12-07

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MEXP-1524-analysis-methods.tsv	Tabular
	E-MEXP-1524-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-1524-configuration.xml	Xml
	E-MEXP-1524-percentile-ranks.tsv	Tabular
	E-MEXP-1524.condensed-sdrf.tsv	Tabular

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E-GEOD-62315
E-MEXP-1282

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Defense signaling in potato

Project description:Potato cultivar Russet Norkotah was grown in a growth chamber at 16 hour light/8 hour dark at 22°C fertilized with Osmocote. Plants were from tissue culture, transplanted to soil and used approximately 4 weeks after transplanting. RNA was extracted from leaves using Trizol. Plants were sprayed with one of four treatments, either 1mM salicylic acid, 100 µM methyl jasmonate, 1 mg/ml arachidonic acid or 100 µM methyl jasmonate plus 1 mM salicylic acid together. Samples were harvested at 3, 24 and 72 hours after spraying. Each salicylic acid or arachidonic acid treatment has 3 biological replicates done in parallel, using leaves pooled from 2 plants. The control for SA or AA treated plants were water sprayed plants, from which leaves of 3 different plants were pooled at each timepoint, done side-by-side with the treated samples. Methyl jasmonate or MJ+SA treatments had 2 biological replicates each, using leaves pooled from 2 plants. A separate set of water sprayed plants (from the SA and AA control treatments) were used as controls, and pooled leaves from 3 plants were used at each time-point, and done in parallel with the treated samples. Keywords: Direct comparison

2007-09-01 | GSE8208 | GEO

RNA-seq of Arabidopsis thaliana seedlings treated with MeJA or BTH

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A Vitis vinifera WRKY transcription factor, VvWRKY1, enhances Plasmopara viticola resistance in transgenic grapevines through activation of jasmonic acid signalling pathway

Project description:WRKY genes are transcription factors involved in plant response to pathogen attacks in many plant species. These proteins have been shown to activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. To understand the molecular mechanisms involved in grapevine defence, we previously identified a WRKY gene, VvWRKY1, which was able to enhance tolerance to fungal pathogens when overexpressed in tobacco. To elucidate its role in grapevine, we generated transgenic grapevines that overexpress VvWRKY1. Microarray analyses were performed to compare global gene expression in leaves of the transgenic and wild-type lines. Results showed that expression of genes encoding defence-related proteins was enhanced in the transgenic 35S::VvWRKY1 line. Quantitative RT-PCR analysis confirmed that three genes putatively involved in jasmonic acid signalling pathway, two genes encoding JASMONATE ZIM-domain (JAZ) proteins and one lipoxygenase, are over-expressed. The ability of VvWRKY1 to trans-activate their corresponding promoters was confirmed by transient expression assay in grape protoplasts. After challenging with the downy mildew pathogen Plasmopara viticola, resistance was enhanced in the transgenic line compared to the wild-type line. These results suggest that VvWRKY1 transcription factor is able to control plant disease resistance to one of the main grapevine pathogen by activating jasmonic acid signalling pathway in grapevine.

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Pearl millet seedlings infected with rust, or treated with MeJA or SA

Project description:This SuperSeries is composed of the following subset Series: GSE13478: Pearl millet seedlings treated with methyl jasmonate (MeJA) GSE13479: Pearl millet seedlings infected with rust (Puccinia substriata) GSE13480: Pearl millet seedlings treated with salicylic acid (SA) Refer to individual Series

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Project description:The specificity and synergisms of methyl jasmonate and cyclodextrin elicitor effects in grapevine were analysed at the transcrptomic level. To this end, 12-14 day old cell cultures established from Vitis vinifera L. cv Monastrell calli were elicited with 50 mM cyclodextrin (CD), 100 µM methyl jasmonate (MJ) or the combination of both elicitros (MJ+CD). The experiment was carried out in triplicate and untreated cell cultures were followed in parallel as a control. Cells of each culture were isolated just prior and 24h after the treatment to obtain RNA. GrapeGen GeneChip microarray hybridization was performed from each sample. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Pablo Carbonell-Bejerano. The equivalent experiment is VV44 at PLEXdb.]

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2020-01-27 | E-MTAB-8697 | biostudies-arrayexpress

Transcription profiling of grapevine cell culture treated with methyly jasmonate, salicylic acids and ethanol controls

Project description:Calli generated from grape berries were used to establish a cell suspension in Gamborgs B5 liquid medium with minimal organics supplemented with 30 gL-1 sucrose, 0.25 gL-1 casein hydrolysate, 0.93 ?M kinetin and 0.54 ?M naphthaleneacetic acid (NAA) and incubated in darkness at 26°C. <br>The cells were cultured in 250 mL flasks (50 mL of cell suspension in each) incubated in darkness on an orbital shaker at 100 rpm. The cell suspensions were sub-cultured every 2 weeks using an initial packed cell volume (PCV) of 15%. For the evaluation of the effect of elicitor addition on the changes in gene expression, 100 mL flasks containing 20 mL of the above cell suspension were used. Each treatment was conducted in triplicate.<br><br>In a set of preliminary experiments studying the effect of elicitors on the volatiles produced by the cell suspensions, the compounds were added during the exponential growth phase when the PCV was approximately 50%. After 72h incubation in darkness after elicitor addiction, cells were harvested. The elicitors screened for their ability to induce volatile compound production were: 500 µM methyl jasmonate (MeJA); 500 uM MeJA + 500 µM salicylic acid (SA); 500 µM jasmonic acid (JA). Controls were conducted by adding the same volume of the solvent used to dissolve each elicitor to the cultures. For the jasmonate and SA experiments the solvent was ethanol.<br>

2009-04-28 | E-MEXP-1524 | biostudies-arrayexpress

Genes activated by s.a through the activity of phosphatidylinositol 4-kinase- Role of phosphatidylinositol 4-kinase

Project description:Cell suspension culture of Arabidopsis thaliana (ecotype Columbia) was treated by 250 uM salicylic acid and by two different concentrations of wortmannin (1 uM and 30 uM) for 4 hours. Keywords: dose response,treated vs untreated comparison

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