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E-MEXP-1703

ABSTRACT: Transcription profiling of Arabidopsis seedlings treated with glucose and indole-3-acetic acid (auxin) to study glucose-auxin interactions

PROVIDER: E-MEXP-1703 | ExpressionAtlas | 2015-04-02

REPOSITORIES: ExpressionAtlas

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Arabidopsis thaliana: auxin-treated seedlings versus control seedlings

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Arabidopsis thaliana: auxin-treated seedlings versus control seedlings

Project description:Transcriptional profiling of Arabidopsis thaliana seedlings treated with auxin (indole-3-acetic acid), highlighting to the physiological function of auxin by observing early response of gene expressions in Arabidopsis seedlings. Two-condition experiment, auxin-treated seedlings vs. control seedlings. Biological replicates:2 control replicates, 2 auxin-treated.

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Project description:21120 Arabidopsis thaliana gene sequence tags were amplified and purified using a novel bead-based strategy (see Publication for more details).This experiment was carried out to demonstrate the use of these arrays for expression profiling of Arabidopsis thaliana.Briefly, 10-day old Arabidopsis seedlings were treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin) and the effects on gene expression were analysed.

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