E-MEXP-2144
Ontology highlight
ABSTRACT:
PROVIDER: E-MEXP-2144 | ExpressionAtlas | 2014-07-03
REPOSITORIES: ExpressionAtlas
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| E-MEXP-2144-analysis-methods.tsv | Tabular | |||
| E-MEXP-2144-atlasExperimentSummary.Rdata | Rdata | |||
| E-MEXP-2144-configuration.xml | Xml | |||
| E-MEXP-2144-percentile-ranks.tsv | Tabular | |||
| E-MEXP-2144-zoomifications-log.tsv | Tabular |
Items per page: 5 1 - 5 of 63 |
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Project description:<p><strong>BACKGROUND:</strong> Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected distal leaves following an initial localized infection. However, little information is available on the molecular biological basis of SAR especially in uninfected distal leaves. </p><p><strong>METHODS:</strong> Here, we used two SAR-inducing pathogen to induce SAR in Arabidopsis, and a metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) and mass spectrometry (MS) technique was used to identify SAR-related metabolites in SAR-inducing pathogen infected local leaves and uninfected distal leaves. </p><p><strong>RESULTS:</strong> Multiple statistical analyses were used to identify differentially expressed metabolites. The result showed that primary metabolism and secondary metabolism were significantly altered in local leaves and distal leaves, including phenolic compounds, amino acids, nucleotides, organic acids and many other metabolites.</p><p><strong>CONCLUSIONS:</strong> The content of amino acids and phenolic compounds increased in distal leaves, suggesting their contribution to the establishment of SAR in distal leaves. In addition, 2’-hydroxy-4, 4’, 6’-trimethoxychalcone, phenylalanine and p-coumaric acid were identified as potential components which may play important roles both in basic resistance and SAR. This study provided a reference for understanding metabolic mechanism associated with SAR in Arabidopsis, which will be useful for further investigation of the molecular basis of SAR.</p>
2020-05-14 | MTBLS1550 | MetaboLights
Project description:Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected distal leaves following an initial localized infection. However, little information is available on the molecular biological basis of SAR especially in uninfected distal leaves. Here, we used two SAR-induced bacteria to induce SAR in Arabidopsis, a metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) and mass spectrometry (MS) technique was used to identify SAR-related metabolites in SAR-induced bacteria infected local leaves and distal leaves of locally SAR-induced bacteria infected plants. Multiple statistical analyses were used to identify differentially expressed metabolites (DEMs). The result showed primary metabolism and secondary metabolism were significantly altered in local leaves and distal leaves, including phenolic compounds, amino acids, nucleotides, organic acids and many other metabolites. Furthermore, the content of amino acids, phenolic compounds, coenzymes and its derivatives increased in distal leaves, suggesting these metabolites contributed to the establishment of SAR. In addition, 2’-hydroxy-4,4’,6’-trimethoxychalcone, phenylalanine and ethyl docosahexaenoate were identified as potential components which may play important role both in basic resistance and SAR. This study provided a reference for understanding metabolic mechanism associated with SAR in Arabidopsis, which will be useful for further investigation of the molecular basis of SAR.
2020-05-05 | PXD017073 |
Project description:Goal of this study was to determine changes in transcription profile in mock versus G3P treated plants. Arabidopsis (Col-0 ecotype) plants were infiltrated with petiole exudate, with or without G3P, and distal leaves were sampled 24 h post treatments. Keywords: Glycerol-3-Phosphate, Exudate, Salicylic acid, Arabidopsis, Systemic Acquired Resistance, Defense
2011-03-15 | GSE26973 | GEO
Project description:Systemic Acquired Resistance (SAR) as a plant immune response improves immunity of distal tissue after local exposure to a pathogen. Stomata pores on leaf surfaces are formed by guard cells that recognize bacterial pathogens via pattern recognition receptors. We found that Arabidopsis thaliana plants that have been previously exposed to the pathogenic bacteria Pseudomonas syringae pv. tomato DC3000 (Pst) exhibit an altered stomatal response compared to control plants when systemic leaves are later exposed to the bacteria. Reduced stomatal apertures of SAR primed plants lead to decreases in the number of bacteria that enter the apoplastic space of the leaves. To examine how SAR affects molecular processes in guard cells of distal leaves, we collected Arabidopsis guard cell samples and extracted lipids, metabolites and proteins using a 3-in-1 method. Multi-omic results have revealed molecular components of SAR response specific to the functions of guard cells and roles of ROS and fatty acid signaling in guard cell SAR response. Additionally, our results show an increase in palmitic acid and its derivative 9-PAHSA in primed guard cells. Palmitic acid may play a role as an agonist to Flagellin Sensitive 2 (FLS2), which initiates stomatal closure upon perception of bacterial flagella. The improved understanding of how SAR immune signals affect stomatal movement and immunity can aid biotechnology and marker-based breeding of crops for enhanced disease resistance.
2021-09-09 | PXD022880 | Pride
Project description:Systemic transcriptional responses in Arabidopsis thaliana distal leaves to wounding
2010-04-22 | E-MEXP-2144 | biostudies-arrayexpress
Project description:Transcriptional profiling of Arabidopsis thaliana, comparing control wild-type (ecotype Wassilewskija, Ws) leaves with leaves from transgenic plants overexpressing the transcription factor RAP2.6L under control of the cauliflower mosaic virus 35S promoter (RAP2.6L-OX; this line was originally described in Krishnaswamy et al (2010)).
2017-06-30 | GSE90601 | GEO
Project description:Exploring miRNA-related antisense transcription in Arabidopsis through RNA transcript profiling of smRNA pathway-defective mutants on a custom high-resolution oligonucleotide array.
2009-04-10 | GSE15199 | GEO
Project description:Transcriptional profiling of Arabidopsis leaves comparing mock-treated leaves with Botrytis cinerea infected leaves over a time-course (12 and 24 hrs).
2011-03-01 | GSE24445 | GEO
Project description:<p><strong>BACKGROUND:</strong> Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected distal leaves following an initial localized infection. However, little information is available on the molecular biological basis of SAR especially in uninfected distal leaves. </p><p><strong>METHODS:</strong> Here, we used two SAR-inducing pathogen to induce SAR in Arabidopsis, and a metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) and mass spectrometry (MS) technique was used to identify SAR-related metabolites in SAR-inducing pathogen infected local leaves and uninfected distal leaves. </p><p><strong>RESULTS:</strong> Multiple statistical analyses were used to identify differentially expressed metabolites. The result showed that primary metabolism and secondary metabolism were significantly altered in local leaves and distal leaves, including phenolic compounds, amino acids, nucleotides, organic acids and many other metabolites.</p><p><strong>CONCLUSIONS:</strong> The content of amino acids and phenolic compounds increased in distal leaves, suggesting their contribution to the establishment of SAR in distal leaves. In addition, 2’-hydroxy-4, 4’, 6’-trimethoxychalcone, phenylalanine and p-coumaric acid were identified as potential components which may play important roles both in basic resistance and SAR. This study provided a reference for understanding metabolic mechanism associated with SAR in Arabidopsis, which will be useful for further investigation of the molecular basis of SAR.</p>
2020-06-30 | MSV000085657 | GNPS
Project description:Arabidopsis plants were challenged with Pieris rapae larvae for 3-5 h and then left unchallenged for 24 hr. Local (damaged) and distal (undamaged) leaves were harvested
2004-11-01 | E-MEXP-163 | biostudies-arrayexpress