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E-MEXP-2657

ABSTRACT: Transcription profiling by array of human stromal cells cultured with or without 3D BPH-1 prostate epithelial cells

PROVIDER: E-MEXP-2657 | ExpressionAtlas | 2025-01-22

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MEXP-2657-analysis-methods.tsv	Tabular
	E-MEXP-2657-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-2657-configuration.xml	Xml
	E-MEXP-2657-percentile-ranks.tsv	Tabular
	E-MEXP-2657.condensed-sdrf.tsv	Tabular

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Transcription profiling by array of human stromal cells cultured with or without 3D BPH-1 prostate epithelial cells

Project description:BPH-1 prostate epithelial cells when cultured in Matrigel form 3D acini which closely recapitulate the tissue architecture of the prostate. The presence of primary stroma increases epithelial adhesions and causes changes in morphology. To identify the stromal signals which control actin/cadherin dynamics in epithelial cells we performed a microarray analysis on the stroma using Affymetrix U133 plus 2.0 arrays. Primary stroma from 7 different patients were cultured with (PSE) or without (PS) 3D BPH-1 epithelial acini to determine the gene expression changes within the stroma. The RNA from the Primary epithelia and stromal cultures were used at passages 1 or 1-3, respectively.

2010-06-09 | E-MEXP-2657 | biostudies-arrayexpress

E-MTAB-8510

Project description:RNA-seq of human mesenchymal stromal cells cultured in PEG hydrogels with and without FGF-2

2025-01-16 | E-MTAB-8510 | ExpressionAtlas

E-GEOD-15947

Project description:Transcription profiling by array of human prostate epithelial cells after treatment with 1,25-dihydroxyvitamin D3

2025-01-24 | E-GEOD-15947 | ExpressionAtlas

Gene expression in human basal, luminal, and stromal cell populations from benign prostatic hyperplasia tissues and comparisons with cultured basal cells.

Project description:The understanding of common prostatic disorders has been restricted by both cellular heterogeneity and the scarcity of established cell lines, although organoid technology, based on primary cultures, promises much for the future. In particular, little is known about the aetiology of benign prostatic hyperplasia (BPH), for which culture of single cell types might not accurately not reflect the stromal and epithelial overgrowths observed in tissues. To address the applicability of primary cell culture models of prostate disease, we compared cell type-specific mRNA expression patterns in BPH tissues and primary basal cells cultured from the same transurethral biopsies.

2017-05-09 | E-MTAB-5647 | biostudies-arrayexpress

E-MEXP-1958

Project description:Transcription profiling by array of human dendritic cells co-cultured with carcinoma cells or treated with lipopolysaccharide

2025-01-22 | E-MEXP-1958 | ExpressionAtlas

Comparison of Normal Prostate Fibroblasts Overexpressing Cyclin D1 and Carcinoma Associated Fibroblasts

Project description:The cyclin D1 oncogene encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the Rb protein and promotes progression through G1 to S phase of the cell cycle. Several prostate cancer cell lines and a subset of primary prostate cancer samples have increased cyclin D1 protein expression. However, the relationship between cyclin D1 expression and prostate tumor progression has yet to be clearly characterized. This study examined the effects of manipulating cyclin D1 expression in either human prostatic epithelial or stromal cells using a tissue recombination model. The data showed that overexpression of cyclin D1 in the initiated BPH-1 cell line increased cell proliferation rate, but did not elicit tumorigenicity in vivo. However, overexpression of cyclin D1 in Normal Prostate Fibroblasts (NPF) that were subsequently recombined with BPH-1 did induce malignant transformation of the epithelial cells. The present study also showed that recombination of BPH-1 + cyclin D1 overexpressing fibroblasts (NPF cyclin D1) resulted in permanent malignant transformation of epithelial cells (BPH-1 NPF-cyclin D1 cells) similar to that seen with Carcinoma Associated Fibroblasts (CAFs). Microarray analysis showed that the expression profiles between CAFs and NPF cyclin D1 cells were highly concordant including cyclin D1 upregulation. These data indicated that the tumor-promoting activity of cyclin D1 may be tissue-specific. Keywords: cyclin D1; stromal-epithelial interactions; prostate cancer; cDNA microarray

2007-09-01 | GSE6936 | GEO

E-MEXP-1072

Project description:Transcription profiling by array of human A549 epithelial cells infected with poliovirus

2025-01-22 | E-MEXP-1072 | ExpressionAtlas

E-MEXP-1342

Project description:transcription profiling by array mouse mammary epithelial cells treated with TGF-beta to induce epithelial-to-mesenchymal transition

2015-10-22 | E-MEXP-1342 | ExpressionAtlas

E-MEXP-1599

Project description:Transcription profiling by array of human A549 lung epithelial cells exposed to acrolein

2025-01-22 | E-MEXP-1599 | ExpressionAtlas

E-GEOD-40370

Project description:Transcription profiling by array of colonic epithelial cells from mice starved, subsequently refed with or without antibiotics metronidazol and vancomycin

2014-10-10 | E-GEOD-40370 | ExpressionAtlas

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