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E-MEXP-509

ABSTRACT: Transcription profiling by of Arabidopsis leaves after infection with Potyvirus turnip mosaic virus

PROVIDER: E-MEXP-509 | ExpressionAtlas | 2015-10-22

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MEXP-509-analysis-methods.tsv	Tabular
	E-MEXP-509-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-509-configuration.xml	Xml
	E-MEXP-509-percentile-ranks.tsv	Tabular
	E-MEXP-509.condensed-sdrf.tsv	Tabular

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potyvirus-Transcriptional analysis of Arabidopsis thaliana infected by a potyvirus.

Project description:ra03-02_potyvirus - potyvirus - Identification of genes involved in plant/virus interactions. - In this experiment, Arabidopsis plants infected by a virus, Tobacco etch virus (TEV), a potyvirus, were compared with healthy plants to identify genes for which the expression is modified by the viral infection. Analysis of both inoculated leaves and upper young leaves were performed 7 days after the inoculation with the virus (or with only buffer for the healthy plants). Keywords: normal vs disease comparison

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Project description:In this experiment, Arabidopsis plants infected by a virus, Tobacco etch virus (TEV), a potyvirus, were compared with healthy plants to identify genes for which the expression is modified by the viral infection. Analysis of both inoculated leaves and upper young leaves were performed 7 days after the inoculation with the virus (or with only buffer for the healthy plants).

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Project description:A model system of Potyvirus turnip mosaic virus and Arabidopsis was used in this experiment. GFP-tagged virus supplied a visualized marker for us to localize the viral infection foci and its expansion on leaf under UV light. Initially, we dissect an individual infection focus and its adjacent region into four parts and define those four parts as zone 0, 1, 2, and 3, which represented different viral infection stages respectively. Corresponding fours parts were also dissected from control plant treated with turnip leaf sap only. This process was replicated three times totally.

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Project description:Transcriptional profiling of Arabidopsis thaliana, comparing control wild-type (ecotype Wassilewskija, Ws) leaves with leaves from transgenic plants overexpressing the transcription factor RAP2.6L under control of the cauliflower mosaic virus 35S promoter (RAP2.6L-OX; this line was originally described in Krishnaswamy et al (2010)).

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sRNAseq from Arabidopsis leaves non-infected and infected with Turnip mosaic virus (TuMV)

Project description:Sequencing of small RNAs from Arabidopsis leaves non-infected and infected with Turnip mosaic virus at 14 days post inoculation

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