Project description:While it is clear that iron deficiency affects both human and rodent development, and particularly kidney development, the precise pathological changes that occur with iron deficiency have not been distinguished. For example, it is not clear which compartment of the kidney is predominantly affected nor it is clear either which stage of development or which cell type require the most iron. In this study, we used an in vivo mouse model of iron deficiency in order to better understand the targeting of iron in the developing kidney. Mouse kidneys at the embrionic stage E15 were used for the total RNA extraction
Project description:While it is clear that iron deficiency affects both human and rodent development, and particularly kidney development, the precise pathological changes that occur with iron deficiency have not been distinguished. For example, it is not clear which compartment of the kidney is predominantly affected nor it is clear either which stage of development or which cell type require the most iron. In this study, we used an in vivo mouse model of iron deficiency in order to better understand the targeting of iron in the developing kidney.
Project description:To examine whether MFG-E8 deficiency affects gene expression in spleen. Total RNA samples were extracted whole spleen of 4 month old B6 and MFG-E8 deficient mice