Project description:We transfected MEF SEC24D KO cells with SEC24B siRNA for a double KD or combined SEC24s siRNA (SEC24a, 24B and 24C) for a quadruple KD. Cells were transfected with siRNA or Scramble siRNA (control) for 2 days then total RNA for each knockdown sample was extracted and purified for RNA-seq to evaluate the change in transcriptome.
Project description:HEK-293 cells transfected with non-targeting control siRNA or UPF1LL-specific siRNA were treated with vehicle control, puromycin, or thapsigargin as indicated and used for total RNA-seq.
Project description:Flp-In T-REx-293 cells transfected with non-targeting control siRNA or UPF1-specific siRNA as indicated and used for total RNA-seq.
Project description:To investigate the global genes regulated by FTO high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between HepG2 cells transfected with control siRNA or FTO siRNA.
Project description:To investigate the global genes regulated by siIGF2BP3 high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between PC3 cells transfected with control siRNA or siIGF2BP3 siRNA.
Project description:To investigate the global genes regulated by AHR, high-throughput mRNA sequencing (RNA-Seq) was performed to compare the expression profile between DLD-1 cells transfected with control siRNA or AHR siRNA.
Project description:The human HOTAIR-specific siRNA and control siRNA were transfected into MCF-7-TNR cells. RNA was collected at 72 hrs after transfection. RNA-SEQ was carried out to profile the gene expression altered by HOTAIR knockdown.