Project description:We compared gene expression differences in the polytypic species complex Mus musculus (Mus musculus musculus, Mus musculus domesticus, Mus musculus castaneus and Mus musculus ssp) with that of Mus spretus via oligonucleotide microarrays representing more than 20,000 genes. Analysis of the results by two way ANOVA statistics suggests that the most genes with significant differences in expression levels among the subspecies are found in liver and kidney and the least in testis. This picture is different when one compares with Mus spretus, where the largest number of differences is found in testis. The design we employed is a reference design. All tissues were hybridized against a pool of that same tissue from 9 C57BL6 mice. All mice were roughly 12 weeks of age. To control for biological variation, we have used several individual males from each sub-species. RNA was isolated from three different organs, namely brain, liver/kidney and testis.

2010-06-30 | E-GEOD-2597 | biostudies-arrayexpress

Tabula Muris Senis

Project description:Tabula Muris Senis is a single cell transcriptomic atlas of 18 tissues and organs from Mus musculus across the organism’s life span.

2020-04-30 | GSE149590 | GEO

ChIP-Seq analysis of CCCTC-binding factor (CTCF) binding in rodents

Project description:We explored the microevolutionary trends of CTCF binding evolution by preforming ChIP-seq experiments in five closely related Mus strains, subspecies and species: Mus musculus domesticus, Mus musculus castaneus, Mus spretus, Mus caroli and Mus pahari. All experiments were performed in adult male liver samples in 3 biological replicates and with an input control set. Complementary RNA-seq data from this same study have been deposited in ArrayExpress under accession numebr E-MTAB-5768 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5768 ).

2018-03-06 | E-MTAB-5769 | biostudies-arrayexpress

Contrasting patterns of expression divergence in early and late stages of population divergence in mice

2006-12-13 | GSE2597 | GEO