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E-MTAB-4028

ABSTRACT: Transcription profiling by array of Chicken Embryo Fibroblasts (CEF) and DF-1 in response to recombinant chicken chIFN- and infection with Infectious Bursal Disease Virus (IBDV)

PROVIDER: E-MTAB-4028 | ExpressionAtlas | 2015-11-06

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-4028-analysis-methods.tsv	Tabular
	E-MTAB-4028-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-4028-configuration.xml	Xml
	E-MTAB-4028-percentile-ranks.tsv	Tabular
	E-MTAB-4028.condensed-sdrf.tsv	Tabular

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E-MTAB-3711

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Kinetics of host transcription in CEFs productively infected with recombinant fowlpox virus vaccine vector FP9

Project description:Fowlpox virus (here as FP9, a plaque-purified, high passage-attenuated derivative) effectively suppresses induction of the âinnateâ immune responses, notably the Type I interferon system, of the permissive host (chicken). Despite the extensive usage of fowlpox virus as a recombinant vaccine vector in chickens, its immunomodulatory mechanisms remain largely unknown. In this study, a transcriptomic analysis using the Affymetrix GeneChip chicken genome array was performed at the host gene transcription level at 4, 8, 16 and 24 hours post-infection of mock treated and FP9-infected (MOI=5, 2h) chicken embryo fibroblasts (CEF). The experiment was performed in triplicate with three different batches of CEFs. Because fowlpox virus is capable of expressing antigens in mammalian cells, these studies in chicken cells form a baseline for subsequent study of immunomodulation of mammalian innate immune responses.

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Genome-wide comparison of Chicken Embryo Fibroblasts (CEF) and DF-1 in response to recombinant chicken chIFN-Î± and infection with Infectious Bursal Disease Virus (IBDV)

Project description:The spontaneously immortalised chicken DF-1 cell line is rapidly replacing its progenitor primary chicken embryo fibroblasts (CEF) in studies on avian viruses but no comprehensive study has as yet been reported comparing their immune phenotype. We conducted microarray analysis of the DF-1 and CEF, in both normal and stimulated conditions using recombinant chicken chIFN-Î± and the CEF-adapted infectious bursal disease virus vaccine strain PBG98.

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