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E-MTAB-5399

ABSTRACT: Transcription profiling by microarray of mouse B16F10 cells transduced with cDNAs encoding eGFP control, p19Arf, murine interferon-beta (IF-B) or the combination of p19Arf plus IF-B to study the modulated genes

PROVIDER: E-MTAB-5399 | ExpressionAtlas | 2017-02-09

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-5399-analysis-methods.tsv	Tabular
	E-MTAB-5399-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-5399-configuration.xml	Xml
	E-MTAB-5399-percentile-ranks.tsv	Tabular
	E-MTAB-5399.condensed-sdrf.tsv	Tabular

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Transcription profiling by microarray of mouse B16F10 cells transduced with cDNAs encoding eGFP control, p19Arf, murine interferon-beta (IF-B) or the combination of p19Arf plus IF-B to study the modulated genes

Project description:Taking the advantage that B16F10 cells retain the wild-type p53, we introduced the p53 partner into these cells, the p19Arf. Also, in order to induce a immune stimulation in experiments *in vivo*, we introduced the interferon-beta cDNA. This combination induced several benefits when compared to the use of these factors alone, as seen by propidium iodide staining, tunel staining, several gene expression analysis, tumor growth, mice survival, among others. Because of this, we had the interest to study the effects of the combination of p19Arf plus interferon-beta upon gene regulation of B16F10 cells, compared to the effects of these transgenes alone. Previous works from our group that show several benefits of p19Arf plus interferon-beta combination upon B16F10 cells are described in Merkel et al (2010) doi: 10.1186/1471-2407-10-316, Merkel et al (2013) doi:10.1038/cgt.2013.23 and Medrano et al (2016) doi: 10.1007/s00262-016-1807-8 .

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