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E-MTAB-5473

ABSTRACT: RNA-seq of Medicago truncatula A17 and the ethylene insensitive sickle mutant interacting with the fungal root pathogen, Rhizoctonia solani AG8

OTHER RELATED OMICS DATASETS IN: PRJNA369210

PROVIDER: E-MTAB-5473 | ExpressionAtlas | 2020-12-09

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-5473-analysis-methods.tsv	Tabular
	E-MTAB-5473-analytics.tsv	Tabular
	E-MTAB-5473-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-5473-configuration.xml	Xml
	E-MTAB-5473-g3_g1-mvaPlot.png	Other

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RNA-seq of Medicago truncatula A17 and the ethylene insensitive sickle mutant interacting with the fungal root pathogen, Rhizoctonia solani AG8

Project description:This study compares the response of wild type (A17) and ethylene insensitive mutant (sickle) lines of the model legume Medicago truncatula to infection by the root-infecting necrotrophic fungal pathogen, Rhizoctonia solani AG8 (WAC10335). Two time points were taken, 2 and 7 days after inoculation along with corresponding mock-treated controls.

2017-06-06 | E-MTAB-5473 | biostudies-arrayexpress

Ethylene signaling is important for isoflavonoid mediated resistance to Rhizoctonia solani in roots of Medicago truncatula

Project description:The root-infecting necrotrophic fungal pathogen Rhizoctonia solani causes significant disease to all the world’s major food crops. As a model for pathogenesis of legumes we have examined the interaction of R. solani AG8 with Medicago truncatula. RNAseq analysis of the moderately resistant M. truncatula accession A17 and highly susceptible sickle (skl) mutant (defective in ethylene sensing) identified major transcriptional reprogramming early in A17. Responses specific to A17 included components of ethylene signalling, numerous class IX ERF transcription factor family members, reactive oxygen species metabolism and consistent up-regulation of the isoflavonoid biosynthesis pathway. Mass-spectrometry revealed accumulation of the isoflavonoid related compounds liquiritigenin, formononetin, medicarpin and biochanin A in A17. Over-expression of an isoflavone synthase (IFS) in M. truncatula roots increased isoflavonoid accumulation and resistance to R. solani. Addition of exogenous medicarpin suggested this phytoalexin may be one of several isoflavonoids required to contribute to resistance to R. solani. Together these results provide evidence for the role of ethylene-mediated accumulation of isoflavonoids during defence against root pathogens in legumes.

2017-06-06 | GSE94260 | GEO

RNA-seq of Medicago truncatula A17 and the ethylene insensitive sickle mutant interacting with the fungal root pathogen, Rhizoctonia solani AG8.

Project description:RNA-seq of Medicago truncatula A17 and the ethylene insensitive sickle mutant interacting with the fungal root pathogen, Rhizoctonia solani AG8.

| PRJEB19425 | ENA

Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum

Project description:affy_ralstonia_medicago - Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease. Its infection process was studied with an in vitro inoculation procedure on intact roots of Medicago truncatula. The pathosystem involved susceptible A17 and resistant F83005.5 M truncatula lines infected with the pathogenic strain GMI1000. The mutant A17 line, Sickle, which showed a resistant phenotype was also part of the experiment. To identify host signaling pathway triggered by R. solanacearum infection with a focus on the involvment of ethylene, we used the Medicago Affymetrix array to monitore the expression profiles and the molecular process associated with initial symptoms development (12hpi) and colonization (72hpi). In order to maximize chances to observe differential gene expression, RNA samples were extracted from the root infection zone (root tips) -Three Medicago truncatula lines, A17, F83005.5 and sickle were inoculated with GMI1000 Ralstonai solanacearum strain (107 cfu/ml). RNA were extracted from root extremities (1 cm above the root tip) at time 0, 12h and 72h post inoculation. Three biological repeats were conducted normal vs disease comparison, time course, 27 arrays - Medicago

2011-09-09 | E-GEOD-18473 | biostudies-arrayexpress

Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum

2011-09-10 | GSE18473 | GEO

E-MTAB-3966

Project description:Transcription profiling by high throughput sequencing of grapevine wood infected by a fungal pathogen in the presence of a root biological control agent

2019-05-14 | E-MTAB-3966 | ExpressionAtlas

affy_infection_medicago-Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the Mtnf-ya1-1 mutant

Project description:affy_infection_medicago - affy_infection_medicago - MtNF-YA1 is a transcription factor involved in the regulation of nitrogen fixing nodule organogenesis. Mtnf-ya1-1 mutant has been shown to be more resistant to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses, 1 and 6 days post inoculation (dpi) between WT and the Mtnf-ya1-1 mutant, in order to identify MtNF-YA1-dependent gene networks during infection. Data about non inoculated A17 control plants and infected A17 at 1 dpi have been already obtained in another experiment in which the conditions of in vitro culture of M. truncatula plants and ofA.euteiches(Ae) inoculation were exactly the same than the ones used in these experiments.-Fifteen-day-old A17 or Mtnf-ya1-1 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one or six days after inoculation. Three independent repeats were performed.

2011-11-01 | GSE26046 | GEO

Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) and NFP allelic mutants

Project description:affy_aphanomyces_medicago - NFP is one of the putative Nod factor receptor and plays therefore a key role in the establishment of symbiosis. nfp allelic mutants are more susceptible to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare the early plant responses, 1 day post inoculation (dpi) between WT and the nfp2 mutant, in order to identify NFP-dependent gene networks during infection.-Fifteen-day-old A17 or nfp-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one day after inoculation. Three independent repeats were performed. Keywords: normal vs ems mutant comparison,near isogenic lines (hif)

2011-09-02 | GSE20587 | GEO

affy-agro-bi-medicago-AGRO-BI-Toulouse

Project description:affy_agro-bi_medicago - Identification of genes from the model legume Medicago truncatula whose expression is affected by the plant nitrogen status, with or without inoculation with the symbiotic bacteria Sinorhizobium meliloti. - Comparison of the supernodulant, nitrogen-insensitive, sunn-2 mutant with the A17 wild type genotype.-The plant root systems of plants were split into two parts, each one being installed in a separate compartment. For the “S” treatment, one part was supplied with 10 mM NH4NO3 while the other part was supplied with a nitrogen-free nutrient solution. For the “L” treatment, one part was supplied 0.5mM NO3- and the other part was supplied with the nitrogen free solution. Eight biological materials (designated AL, AS, SL, SS, IL, IS, NIL, NIS), with three biological repeats for each, were collected and analyzed. The effects of the S and L conditions were investigated on wild-type A17 (AL vs. AS) and sunn-2 mutant plants (SL vs. SS); one set of A17 plants was inoculated with Sinorhizobium meliloti (IL vs. IS), harvested at four days post inoculation and compared to non-inoculated plants (NIL vs. NIS). Keywords: growth in nitrogen-sufficient (s) vs. nitrogen-limited (l) conditions

2010-10-28 | GSE17253 | GEO

affy_infection_medicago-Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the hap2a-1 mutant

Project description:affy_infection_medicago - affy_infection_medicago - HAP2a is a transcription factor involved in the regulation of nitrogen fixing nodule organogenesis. hap2a-1 mutant has been shown to be more resistant to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses, 1 and 6 days post inoculation (dpi) between WT and the hap2a-1 mutant, in order to identify HAP2a-dependent gene networks during infection. Data about non inoculated A17 control plants and infected A17 at 1 dpi have been already obtained in another experiment in which the conditions of in vitro culture of M. truncatula plants and ofA.euteiches(Ae) inoculation were exactly the same than the ones used in these experiments.-Fifteen-day-old A17 or hap2a-1 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one or six days after inoculation. Three independent repeats were performed. 12 arrays - Medicago; normal vs disease comparison,normal vs ems mutant comparison,time course

2011-11-01 | E-GEOD-26046 | biostudies-arrayexpress

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