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E-MTAB-5671

ABSTRACT: RNA-seq of E10.5 limb mesenchymal cells from mice with mutations in Mycn and mir17-92

PROVIDER: E-MTAB-5671 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-5671-analysis-methods.tsv	Tabular
	E-MTAB-5671-analytics.tsv	Tabular
	E-MTAB-5671-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-5671-configuration.xml	Xml
	E-MTAB-5671-g2_g3-mvaPlot.png	Other

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RNA-seq of E10.5 limb mesenchymal cells from mice with mutations in Mycn and mir17-92

Project description:RNA-seq of E10.5 limb mesenchymal cells from mice with mutations in Mycn and mir17-92

| PRJEB20972 | ENA

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Project description:Mice with conditionally deactivating mutations in Mycn (Mycnfl/fl) or mir17-92 & mir106b (mir17-92fl/fl ; mir106b-/-) were crossed with mice expressing Cre-recombinase driven by the Prx1 promoter (Prx1Cre). Embryos were collected at E10.5, and distal compartment of hind- and fore-limbs were isolated from wild-type and conditional knock-out embryos (n=3 replicates per biologic group). Total RNA was extracted from each specimen with Trizol, and used in RNA-seq library preparation with Illumina Truseq mRNA library kit. Libraries were sequenced on the Illumina NextSeq system with 75bp single end reads.

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